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We take your existing hybridoma cell line and produce monoclonal antibodies for you either by in vivo ascites or in vitro tissue culture. Purification of the monoclonal antibody is accomplished by Protein A/G chromatography. Ascites may contain nonspecific antibodies from the animal. Although individual monoclonal production rates cannot be predicted, ascites production is generally about 1mg/mL ascites and 3-5 mL per mouse. To request your monoclonal antibody production, please contact us.
Monoclonal Production Process | Time (est. days) | | Obtain cryopreserved cells from your source or cell library | | | Tissue culture expansion and Cryopreservation of backup cultures | 7 | | Ascites production (10 mice) | 14 |
Tissue culture production (1L) | 14 | | Purification of Monoclonal Antibody | 7 | | Cell Banking | Annual |
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