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Read below for a question regarding our Green Cathepsin B Assay:
Question: Is it necessary to predilute 1:5 in water? Can I just dilute it 1:250 into the staining solution?
Answer: It is not necessary to predilute 1:5 your 250X stock in water then perform the 1:50 dilution after but it is recommended for better solubility of the dye and ease/accuracy of pipetting in downstream serial dilution. If you wish to directly dilute your stock for 1:250, you can do so. Just remember your end goal is to reach 1X final concentration of the dye in the cell suspension.
We hope the above information is helpful!
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