Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody (M268)

Name: Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: JM2681
Price: 329.0 USD
Availability: InStock

Our JNK (Thr-183/Tyr-185) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat JNK (Thr-183/Tyr-185) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of JNK in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal JNK (C-terminal region) (Left) or mouse monoclonal JNK (Thr-183/Tyr-185) (Right). The antibodies were detected using goat anti-mouse DyLight® 594.

Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-JNK1 (lanes 1 & 2) or anti-JNK1 (T183/Y185) (lanes 3 & 4).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: JM2681

Volume: 100 µL

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Price:

$329.00

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Product Details

Target

JNK (Thr-183/Tyr-185)

Target Description

The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1, 2, 3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2 and other transcription factors.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M268

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

MAPK8

Molecular Weight

46/54

Antigen

Clone M268 was generated from a dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to the conserved site in rat and mouse JNK1 and JNK2.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects 46 and 54 kDa* proteins corresponding to the apparent molecular mass of JNK1 and JNK2 on SDS-PAGE immunoblots of human A431 and HeLa cells, as well as rat PC12 cells treated with calyculin A.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SAPK1, p49MAPK, MAPK, Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, Stress-activated protein kinase JNK1, Stress-activated protein kinase 1c (SAPK1c), JNK46

UniProt Details

UniProt (Human): P45983
UniProt (Immunogen Species): P45983

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Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody (M268)

SKU: JM2681

Product Details

Bulk Order Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody

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