Our Laminin β2/γ1 Subunits Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Laminin β2/γ1 Subunits Antibody and is Protein A purified. It is great for use in WB(Nat), ICC.
Native western blot image of human laminin isoforms: laminin 521 (α5β2γ1), laminin 121 (α1β2γ1), laminin 221 (α2β2γ1), laminin 332 (α3β3γ2), laminin 511 (α5β1γ1), laminin 411 (α4β1γ1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin β2/γ1 subunit (LM0461) at 1:1000.
The human basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850 kDa) oligomer, consisting of three different chains laminin alpha (α), beta (β), and gamma (γ) joined by disulfide bonds. The structure of human laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain including 5 alpha chains, 4 beta chains, and 3 gamma chains. Laminin β2 and γ1 are found in laminin 121, laminin 221, laminin 421, and laminin 521. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.
Protein A Purified
Monoclonal
M046
IgG2b
ICC, WB
Mouse
LAMC1
760
Clone (M046) was generated from a recombinant human laminin 521 protein that includes a heterotrimer of three subunits laminin α5, laminin β2, and laminin γ1 subunits.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:200
Unconjugated
Clone M046 detects native laminin isoforms that include both laminin β2 and γ1 subunits. In native immunoblots, the antibody detects laminin 521, laminin 221, and laminin 121, but does not detect lamin 332 and laminin 411. The antibody weakly detects laminin 511. This antibody can be used for immunocytochemical detection of laminin β2/γ1 subunits, and can also be used for detection of native laminin isoforms in immunoblots and ELISA.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
