Anti-MuRF1 (C-terminal region) Antibody

Name: Anti-MuRF1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: MP3401
Price: 329.0 USD
Availability: InStock

64 Citations

Our MuRF1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MuRF1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).

Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).

Western blot analysis of MuRF1 (cat. MP3401), Atrogin-1 (cat. AP2041), FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907

Western blotting analysis of the expression of Atrogin-1 (cat. AP2041, 1:1000) and MuRF1 (cat. MP3401, 1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807

Western blot showing MuRF-1( cat MP3401, 1:500) and atrogin-1 (cat. AP2041), 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400

Markers for muscle degeneration, Myostatin and MuRF-1 (cat. MP3401, 1:1000), were significantly less expressed in CTMP-KO gastrocnemius muscle compared to WT mice. Image from publication CC-BY-4.0. PMID: 36652767

The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1 (cat. AP2041), and MuRF1 (cat. MP3401) in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022

Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Go to Full Product Details

SKU: MP3401

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

MuRF1 (C-terminal region)

Target Description

Muscle proteolysis is regulated by the ATP-dependent ubiquitin–proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFα treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

TRIM63

Molecular Weight

30/38

Antigen

MuRF1 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:300

Conjugate

Unconjugated

Specificity

The antibody detects 38 and 30 kDa* proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Tripartite motif 63,TRIM63, SMRZ, IRF, RNF28, Muscle RING finger, MURF-1

UniProt Details

UniProt (Human): Q969Q1
UniProt (Immunogen Species): Q969Q1

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30922397	not listed	Liu, D, et al. 2019. IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway. Skeletal muscle, 8.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28762162	1:1000	Sakai, H, et al. 2017. Effect of acute treadmill exercise on cisplatin-induced muscle atrophy in the mouse. Pflügers Archiv : European journal of physiology, 1495-1505.
28286533	1:100	Zhang, Y, et al. 2017. Chinese Herbal Medicine Baoyuan Jiedu Decoction Inhibited Muscle Atrophy of Cancer Cachexia through Atrogin-l and MuRF-2. Evidence-based complementary and alternative medicine : eCAM, 6268378.

Immunohistochemistry: Rat
PMID	Dilution	Publication
32806520	not listed	Rusbana, TB, et al. 2020. Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model. Nutrients, 2409.

Western Blot: Human
PMID	Dilution	Publication
34395422	1:1000	Halle, JL, et al. 2021. The Effect of Mechanical Stretch on Myotube Growth Suppression by Colon-26 Tumor-Derived Factors. Frontiers in cell and developmental biology, 690452.
33238442	1:1000	Oxfeldt, M, et al. 2020. Influence of Fermented Red Clover Extract on Skeletal Muscle in Early Postmenopausal Women: A Double-Blinded Cross-Over Study. Nutrients, 3587.
32191600	not listed	Arentson-Lantz, EJ, et al. 2020. Countering disuse atrophy in older adults with low-volume leucine supplementation. Journal of applied physiology (Bethesda, Md. : 1985), 967-977.
32050585	1:500	Aravena, J, et al. 2020. Angiotensin (1-7) Decreases Myostatin-Induced NF-κB Signaling and Skeletal Muscle Atrophy. International journal of molecular sciences, 1167.
31568675	not listed	Gharahdaghi, N, et al. 2019. Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men. Journal of cachexia, sarcopenia and muscle, 1276-1294.
31518665	not listed	Moro, T, et al. 2019. Low skeletal muscle capillarization limits muscle adaptation to resistance exercise training in older adults. Experimental gerontology, 110723.
31513450	1:1000	Fyfe, J, et al. 2019. Cold water immersion attenuates anabolic signaling and skeletal muscle fiber hypertrophy, but not strength gain, following whole-body resistance training. Journal of applied physiology (Bethesda, Md. : 1985), 1403-1418.
31123725	not listed	Brightwell, C, et al. 2019. Moderate-intensity aerobic exercise improves skeletal muscle quality in older adults. Translational sports medicine, 109-119.
30928279	not listed	Park, YM, et al. 2019. Acute estradiol treatment reduces skeletal muscle protein breakdown markers in early- but not late-postmenopausal women. Steroids, 43-49.
28774889	1:2000	Crossland, H, et al. 2017. A dynamic ribosomal biogenesis response is not required for IGF-1-mediated hypertrophy of human primary myotubes. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 5196-5207.

Western Blot: Mouse
PMID	Dilution	Publication
38792078	not listed	Zhou, Y, et al. 2024. Deciphering the Therapeutic Role of Lactate in Combating Disuse-Induced Muscle Atrophy: An NMR-Based Metabolomic Study in Mice. Molecules, 0.
38569555	not listed	Domaniku-Waraich, A, et al. 2024. Oncostatin M signaling drives cancer-associated skeletal muscle wasting. Cell Reports, 101498.
37525907	not listed	Wang, L, et al. 2023. ER Stress is Activated and Involved in Disuse-Induced Muscle Atrophy. Frontiers in Bioscience, 136.
37699022	not listed	Hsu, TH, et al. 2023. The combination of quercetin and leucine synergistically improves grip strength by attenuating muscle atrophy by multiple mechanisms in mice exposed to cisplatin. PloS one, e0291462.
37526646	1:1000	Schifino, AG, et al. 2023. Resistance wheel running improves contractile strength, but not metabolic capacity, in a murine model of volumetric muscle loss injury. Experimental physiology, .
37344807	not listed	Zhang, He, et al. 2023. Decreased expression of H19/miR-675 ameliorates muscle atrophy by regulating the IGF1R/Akt/FoxO signaling pathway. Molecular Medicine (Cambridge, Mass.), 78.
37257944	1:1000	Sato, K, et al. 2023. Platinum-based anticancer drugs-induced downregulation of myosin heavy chain isoforms in skeletal muscle of mouse. Journal of pharmacological sciences, 167-177.
37237400	1:500	Orozco-Aguilar, J, et al. 2023. Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic flux. Biological research, 28.
37165186	1:1000	Bilgic, S.N., et al. 2023. EDA2R–NIK signalling promotes muscle atrophy linked to cancer cachexia. Nature, 827-834.
36536512	1:1000	Raymond‐Pope, C.J., et al. 2023. Restricted physical activity after volumetric muscle loss alters whole‐body and local muscle metabolism. Journal of Physiology, 743-761.
36652767	1:1000	Wang, J, et al. 2023. Carboxyl-terminal modulator protein (CTMP) deficiency mitigates denervation-induced skeletal muscle atrophy. Biochemical and biophysical research communications, 155-161.
36353482	not listed	Amitani, H., et al. 2022. Impact of Ninjin'yoeito on frailty and short life in klotho-hypomorphic (kl/kl) mice. Frontiers in Pharmacology, 973897.
34270178	1:1000	Fix, DK, et al. 2021. Wheel running improves fasting-induced AMPK signaling in skeletal muscle from tumor-bearing mice. Physiological reports, e14924.
34268902	1:1000	Sakai, H, et al. 2021. Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice. Journal of cachexia, sarcopenia and muscle, 1570-1581.
33803124	1:1000	Paixão, AO, et al. 2021. Palmitic Acid Impairs Myogenesis and Alters Temporal Expression of miR-133a and miR-206 in C2C12 Myoblasts. International journal of molecular sciences, 2748.
33731895	not listed	Yoo, W, et al. 2021. Pancreatic cancer induces muscle wasting by promoting the release of pancreatic adenocarcinoma upregulated factor. Experimental & molecular medicine, 432-445.
33386611	1:2000	Kawanishi, N, et al. 2021. Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice. Muscle & nerve, 600-607.
33113850	1:500	Abrigo, J, et al. 2020. Sarcopenia Induced by Chronic Liver Disease in Mice Requires the Expression of the Bile Acids Membrane Receptor TGR5. International journal of molecular sciences, 7922.
31736871	1:2000	Counts, BR, et al. 2019. The Effect of Estradiol Administration on Muscle Mass Loss and Cachexia Progression in Female Apc Min/+ Mice. Frontiers in endocrinology, 720.
31525347	1:1000	Scholpa, NE, et al. 2019. β2-adrenergic receptor-mediated mitochondrial biogenesis improves skeletal muscle recovery following spinal cord injury. Experimental neurology, 113064.
31514753	1:500	Pérez, V, et al. 2019. The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability. Acta neuropathologica communications, 147.
31126323	not listed	Lala-Tabbert, N, et al. 2019. Targeted ablation of the cellular inhibitor of apoptosis 1 (cIAP1) attenuates denervation-induced skeletal muscle atrophy. Skeletal muscle, 13.
30943315	1:1000	Silva,WJ, et al. 2019. miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes. Acta physiologica (Oxford, England), e13278.
30922397	not listed	Liu, D, et al. 2019. IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway. Skeletal muscle, 8.
30884219	not listed	Zhang, P, et al. 2019. Hemojuvelin is a novel suppressor for Duchenne muscular dystrophy and age-related muscle wasting. Journal of cachexia, sarcopenia and muscle, 557-573.
30681896	not listed	Choi, RH, et al. 2019. TRB3 regulates skeletal muscle mass in food deprivation-induced atrophy. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 5654-5666.
30530483	1:1000	Radigan, K, et al. 2019. Influenza A Virus Infection Induces Muscle Wasting via IL-6 Regulation of the E3 Ubiquitin Ligase Atrogin-1. Journal of immunology (Baltimore, Md. : 1950), 484-493.
29671242	not listed	Shin, K, et al. 2018. Fbxw7β is an inducing mediator of dexamethasone-induced skeletal muscle atrophy in vivo with the axis of Fbxw7β-myogenin-atrogenes. Molecular biology reports, 625-631.
29215198	1:2000	Hardee, J, et al. 2018. Inflammatory signalling regulates eccentric contraction-induced protein synthesis in cachectic skeletal muscle. Journal of cachexia, sarcopenia and muscle, 369-383.
29054406	1:2000	Crossland, H, et al. 2017. A novel puromycin decorporation method to quantify skeletal muscle protein breakdown: A proof-of-concept study. Biochemical and biophysical research communications, 608-614.
29021780	1:1000	Paré, MF, et al. 2017. Chronic 5-Aminoimidazole-4-Carboxamide-1-β-d-Ribofuranoside Treatment Induces Phenotypic Changes in Skeletal Muscle, but Does Not Improve Disease Outcomes in the R6/2 Mouse Model of Huntington's Disease. Frontiers in neurology, 516.
28984836	1:1000	Marzuca-Nassr, GN, et al. 2017. Balanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle Atrophy. Nutrients, 1100.
28364027	not listed	Giusto, M, et al. 2017. Skeletal muscle myopenia in mice model of bile duct ligation and carbon tetrachloride-induced liver cirrhosis. Physiological reports, e13153.
28286533	1:1000	Zhang, Y, et al. 2017. Chinese Herbal Medicine Baoyuan Jiedu Decoction Inhibited Muscle Atrophy of Cancer Cachexia through Atrogin-l and MuRF-4. Evidence-based complementary and alternative medicine : eCAM, 6268378.

Western Blot: Rat
PMID	Dilution	Publication
37016292	1:1000	Kotake, H., et al. 2023. Mechanism for exercise-mediated prevention against muscle wasting on extensor digitorum longus muscle in Spontaneously Diabetic Torii fatty rats. Journal of Physiological Sciences, 5.
35606908	1:1000	Wang, R., et al. 2022. Resveratrol ameliorates muscle atrophy in chronic kidney disease via the axis of SIRT1/FoxO1. Phytotherapy Research, 3265-3275.
35432979	1:1000	Kim, J.H., et al. 2022. Effect of oligonol, a lychee‐derived polyphenol, on skeletal muscle in ovariectomized rats by regulating body composition, protein turnover, and mitochondrial quality …. Food Science and Nutrition, 1184-1194.
34748920	1:1000	Yeo, D., et al. 2022. Protective effects of extra virgin olive oil and exercise training on rat skeletal muscle against high-fat diet feeding. Journal of Nutritional Biochemistry, 108902.
32588343	1:1000	Lawrence, MM, et al. 2021. Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading. GeroScience, 65-83.
32985798	not listed	Knapp, F, et al. 2020. Differential effects of right and left heart failure on skeletal muscle in rats. Journal of cachexia, sarcopenia and muscle, 1830-1849.
32806520	not listed	Rusbana, TB, et al. 2020. Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model. Nutrients, 2409.
32405506	1:1000	Tanabe, J, et al. 2020. Relationship between Urinary Liver-Type Fatty Acid-Binding Protein (L-FABP) and Sarcopenia in Spontaneously Diabetic Torii Fatty Rats. Journal of diabetes research, 7614035.
32233950	1:1000	Lu, X, et al. 2020. Modulation of HOXA9 after skeletal muscle denervation and reinnervation. American journal of physiology. Cell physiology, C1154-C1165.
32125770	1:1000	Lawrence, MM, et al. 2020. Massage as a mechanotherapy promotes skeletal muscle protein and ribosomal turnover but does not mitigate muscle atrophy during disuse in adult rats. Acta physiologica (Oxford, England), e13460.
31670600	1:1000	Van Pelt, DW, et al. 2019. Age-related responses to a bout of mechanotherapy in skeletal muscle of rats. Journal of applied physiology (Bethesda, Md. : 1985), 1782-1791.
30091665	1:1000	Shimkus, K, et al. 2018. Responses of skeletal muscle size and anabolism are reproducible with multiple periods of unloading/reloading. Journal of applied physiology (Bethesda, Md. : 1985), 1456-1467.
29123487	not listed	Fortes, M, et al. 2017. Hypertrophy Stimulation at the Onset of Type I Diabetes Maintains the Soleus but Not the EDL Muscle Mass in Wistar Rats. Frontiers in physiology, 830.
28000044	1:1000	Gerlinger-Romero, F, et al. 2017. Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p. Molecular and cellular biochemistry, 187-199.

Recover password

Create my account

Anti-MuRF1 (C-terminal region) Antibody

SKU: MP3401

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-MuRF1 (C-terminal region) Antibody

Related Products

Recently Viewed