![Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.](http://www.antibodiesinc.com/cdn/shop/files/982_figure_1_{width}x.jpg?v=1709769416)
![Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).](http://www.antibodiesinc.com/cdn/shop/files/982_figure_2_{width}x.jpg?v=1709769416)
ImmunoChemistry Technologies
Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay
Sale priceFrom $205.50
![Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled Aß and stained with SR-DEVD-FMK (#931/932). Macrophages that engulf FITC-Aß fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-Aß and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-Aß but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.](http://www.antibodiesinc.com/cdn/shop/files/931_figure_1_{width}x.jpg?v=1709768704)
ImmunoChemistry Technologies
Red Fluorescent SR-FLICA® Caspase-3/7 (DEVD) Assay Kit
Sale priceFrom $216
![Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 µM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).](http://www.antibodiesinc.com/cdn/shop/files/9149_figure_1_{width}x.jpg?v=1709768859)
ImmunoChemistry Technologies
Red Fluorescent SR-FLICA® Caspase-8 (LETD) Assay Kit
Sale priceFrom $216
![Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.](http://www.antibodiesinc.com/cdn/shop/files/960_figure_1_{width}x.jpg?v=1709768760)
![Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.](http://www.antibodiesinc.com/cdn/shop/files/960_figure_2_{width}x.jpg?v=1709768761)
ImmunoChemistry Technologies
Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit
Sale priceFrom $216