Metabolic and Enzymatic Assays

11 products

Metabolism is vital and composed of complex process (pathways) within organisms to maintain homeostasis. These complex pathways are linked together to create or break down molecules to maintain homeostasis. Imbalances in these pathways can lead to diseases such as neurodegenerative, cancer, and diabetes to name a few. Our line of fluorescent Metabolite assays/kits are easy and convenient to use with greater sensitivity than traditional colorimetric assays.

Our Metabolic Assays to measure metabolites are more sensitive than conventional colorimetric assays.

Our Enzymatic Assays used to measure cellular/tissue enzymes are more sensitive than conventional colorimetric assays.

Showing 1 - 11 of 11 products

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

ImmunoChemistry Technologies Fluoro NAD/NADH Detection Kit by Cell Technology

From $591

Figure 1. (a) Fluorescent responses of NP3 (5 μM) toward various analytes (10 μM). Data shown represent fluorescent intensity at 470nm, 30 min after addition of the analytes. (b) ONOO− (final 10 μM) was quickly injected into a solution of NP3 (final 5μM), and the fluorescent intensity at 470 nm was plotted against time. (c) Fluorescence enhancement of NP3 (5μM) at 470 nm as a function of ONOO− (0−10 μM) after 15 min of reaction. All data acquired in PBS (10 mM, pH 7.4) with excitation at 375 nm.

Figure 2. (a) Time-lapse images taken from living EA.hy926 endothelial cells. Cells were preincubated with NP3 (5.0 μM), followed by stimulation with or without SIN-1 (0.5 mM). (b) Dynamic changes of NP3 fluorescence after SIN-1 (0.5 mM) treatment in panel a. (c) Effects of ONOO− scavengers uric acid (100 μM) and FeTTPS (1 μM) on changes in NP3 fluorescence in endothelial cells in the presence of ONOO− (60 μM). PI (red) stains nuclei. NP3 fluorescence was collected at 420−480 nm with λex 405 nm.

ImmunoChemistry Technologies Peroxynitrite Detection Kit

$289

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.

Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.

ImmunoChemistry Technologies Fluorescent Cell Proliferation Assay

From $114

Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent Sarcosine Detection Kit

$357

Figure 2. Phosphate standard curve was generated using fluorometric detection: excitation 535 nm and emission 585 nm. Incubation time = 60 minutes at 37°C. Standard curve range 1.5625 μM to 100μM. Graph was plotted using 4PL non-linear regression.

ImmunoChemistry Technologies Fluorescent Phosphate Detection Kit

$269

ImmunoChemistry Technologies Fluorescent Lactate Detection Kit

$429

ImmunoChemistry Technologies Fluorescent ATP Detection Kit

From $393

ImmunoChemistry Technologies Fluorescent ADP Detection Kit

From $393

Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.

Figure 2. Standard curve (absorbance). Absorbance read-out at 570 nm. R2 =0.9993. Incubation time = 30 minutes. Standard curve range 1.25μM to 80μM. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent Total Cholesterol Detection Kit

$357

Figure 2. Comparison of NADPH & NADH Standard Curves

ImmunoChemistry Technologies Fluorescent NADP/NADPH Detection Kit

From $591

Figure 2. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer and seeded in glass bottom dishes. The cells were then loaded with APF (10μM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters

ImmunoChemistry Technologies Highly Reactive Oxygen Species (hROS) Detection Kit

$289

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Metabolic and Enzymatic Assays

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