ICT’s Block Buffers are specific blockers for antibody-sandwich or antigen-down ELISAs, maintain the water of hydration of dried antigen or antibody coat proteins, minimize non-specific binding interactions during the assay process, increase shelf-life by improving the stability of the coated protein and reduce assay variability.
For example, our General Block contains mammalian protein blocking agents to provide adequate blocking strength for most immunoassays, including monoclonal and polyclonal antibody capture ELISAs and peptide and protein antigen-down ELISAs. This unique blocking buffer contains a heterogeneous mixture of proprietary protein stabilizers and small molecules (including BSA) that block the uncoated regions of the plate. Blocking with ICT’s General Block minimizes non-specific binding interactions during the assay process to reduce background noise and enhance the sensitivity of the assay.
Please see below for relevant products.