FLICA®

16 products

Our FLICA® probes are non-cytotoxic Fluorescent Labeled Inhibitors of CAspases that covalently bind to active caspase enzymes. FLICA® measures the intracellular process of apoptosis instead of a side-effect, such as the turnover of phosphatidyl serine, and eliminates the incidence of false positives that often plagues methods like Annexin V and TUNEL staining. FLICA® can also be used to measure pyroptosis, a highly inflammatory form of programmed cell death.

FLICA® can be used to label suspension or adherent cells and thin tissue sections. After labeling with FAM-FLICA®, cells can be fixed or frozen. For tissues that will be paraffin-embedded after labeling, use our red sulforhodamine SR-FLICA® probes.

We have kits for the detection of: caspase-1 (YVAD or WEHD) (also recognizes caspases 4 and 5), -2 (VDVAD), -3/7 (DEVD), -6 (VEID), -8 (LETD), -9 (LEHD), and -10 (AEVD).

FLICA kits are available with a green, red, or far-red fluorescent label.

Showing 1 - 16 of 16 products

Figure 1. THP-1 cells were treated with either a negative control (A), or PMA (5 ng/mL) followed by LPS (10 ng/mL). Cells were then stained with FAM-YVAD-FMK (cat. 97/98) and analyzed by phase contrast and fluorescence microscopy. In the treated sample, many cells appear bright green, indicating increased caspase-1 activity (B). In the non-induced sample, few green cells are visible, indicating a low level of caspase-1 activity (A). Data courtesy of Dr. Brian Lee, ICT (207:11).

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-1 (YVAD) Assay Kit

From $234

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Flow cytometry analysis of Jurkat cells treated with FLICA® 660-YVAD-FMK. After spiking FLICA® into the cell culture media, the cells were treated with either a negative control (left) or nigericin (20 μM, no.6698) to induce caspase-1 activity (right) for 24 hours. The negative control induced caspase-1 activity in 6.8% of cells (left); treatment with nigericin induced caspase-1 activity in 57.3% of cells (right). This is a ratio of 8:1. Data courtesy of Mrs. Tracy Murphy, ICT, 230-01.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit

$256.50

Figure 1. Flow cytometry analysis of active caspase-9 in MDA-MB-231 (A,B) and ZR-75-1 (C,D) cells. Cells were stained with FAM-FLICA® Caspase-9 reagent. From Figure 5 of R. Krętowski and M. Cechowska-Pasko, The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells, Int J Mol Sci (2022) 23, 9285. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 by the authors.

Flow cytometry analysis of the caspase-9 activity (Assay Kit, Cat no. 912) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 µM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-8 (LETD) Assay Kit

From $216

Figure 1. Cell death in primary rat hippocampal neurons. (A) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (D). DNA is labeled in 4 cells (D), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.

Figure 2. Normal (A) and keratoconus (B) corneal fibroblasts were treated with 200 μM H2O2 for 1 hour, washed, and allowed to recover. Cells were then treated with FAM-DEVD-FMK (cat. 93/94) for 1 hour, then washed with 1X Apoptosis Wash Buffer. Keratoconus corneal fibroblasts treated with H2O2 (B) show a significant increase in caspase-3/7 activity compared to normal cells (A). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, University of California, Irvine.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $216.25

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

$256

Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled Aß and stained with SR-DEVD-FMK (#931/932). Macrophages that engulf FITC-Aß fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-Aß and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-Aß but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $216

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed on a Molecular Devices Gemini XPS 96-well fluorescence plate reader. The excitation spectrum (grey) was generated using an emission of 760 nm. The emission spectrum (red) was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis inducing agent (right), then stained with 660-VAD-FMK (cat. 9120) for 1 hour. Cells were washed twice and read on an Accuri C6 flow cytometer. Treatment with the negative control induced caspase activity in only 8.2% of cells (left), whereas treatment with staurosporine induced caspase activity in 96.5% of cells (right). This is a ratio of 12:1. Data courtesy of Mrs. Tracy Murphy, ICT, 213:48.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Poly (active) Caspase (VAD) Assay Kit

$256.50

Figure 1. HL-60 cells were treated with camptothecin (cat. 6210), followed by SR-VAD-FMK (cat. 916/917) and green FAM-FLISP serine protease inhibitor FFCK (cat. 946), and then analyzed on a scanning laser cytometer. Active caspases stain red (X-axis) and active serine proteases stain green (Y-axis). Co-localization of caspase and serine protease activity is evident in B, C, D, and G. Data courtesy of Dr. J. Grabarek, Brander Cancer Center, NY, and Pomeranian School of Medicine, Szczecin, Poland.

Figure 2. Paramecium were starved for 12-24 hours in 5 mM KCl to induce autogamy. SR-VAD-FMK (cat. 916/917), was added to the live Paramecium for 30 minutes in the dark. Cells were then washed and Hoechst 33342 was added. Cells were incubated in the dark for 10 minutes, washed, and applied to coverslips. Images were captured using a Nikon TE-300 Epi-fluorescent microscope with a SPOT-RT digital camera and merged using SPOT software. Data courtesy of Dr. W. E. Bell, Virginia Military Institute.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Poly (active) Caspase (VAD) Assay Kit

From $216

Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.

Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

Figure 1. Caspase activities in the microglia of white matter injury mice using our FAM-FLICA® kits. Only caspase 1 was significantly upregulated. Figure 1 (d) of L. He et al (2022) miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury, Hindaw Journal of Immunology Research 2022, 1642896. https://doi.org/10.1155/2022/1642896. Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 Liufang He et al.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-6 (VEID) Assay Kit

From $216.25

Figure 1. Monocytes can secrete active caspase-1, therefore cells were labeled with FAM-FLICA® Caspase-1 (WEHD) (cat. 9161/9162) prior to treatment. Cells were then treated with DMSO (control) or 10 µM nigericin (cat. 6698), stained with Hoechst 33342, fixed (cat. 636), and viewed using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Increased levels of FAM-FLICA® staining were seen in the nigericin-treated cells. Data courtesy of Dr. Kristi Strandberg, ICT (235:60).

ImmunoChemistry Technologies Green Fluorescent FAM FLICA® Caspase-1 (WEHD) Assay Kit

From $234

Figure 1. Activity of caspase-8 (using FAM-LETD-FMK) in AGS cells after 24 h incubation with Les-4367 (1 μM) and combined with anti-HER2 antibodies. From Figure 4 of A. Gornowicz et al Multi-Targeting Anticancer Activity of a New 4-Thiazolidinone Derivative with Anti-HER2 Antibodies in Human AGS Gastric Cancer Cells, Int J Mol Sci (2023) 24, 6791. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

Flow cytometry analysis of the caspase-8 activity (Assay kit, Cat. no. 99) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-8 (LETD) Assay Kit

From $216.25

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit

From $216

Flow cytometry analysis of the caspase-10 activity in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit

From $216

Figure 1. Fluorescent Microscopy Analysis of THP-1 Suspension Cells THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

Figure 2. Caspase Activity in Jurkat Cells Jurkat cells (T lymphocytes) were labeled with our Green Fluorescent Poly (active) Caspase FAM FLICA® Inhibitor Reagent (FAM-VAD-FMK) (catalog 92) and viewed under a fluorescence microscope. The grey DIC image (B) reveals five cells in the field of view, but only four of them fluoresce green (A). Four out of five cells are apoptotic and have active caspases present (green). Data courtesy of Dr. Brian W. Lee, ICT.

ImmunoChemistry Technologies Green Fluorescent Poly (active) Caspase FAM FLICA® Assay Kit (FAM-VAD-FMK)

From $216.25

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FLICA®

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