Polyclonal Antibodies

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Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.
Aves Labs Anti-Green Fluorescent Protein Antibody
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Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.Representative section of formalin fixed, paraffin-embedded rat brain showing staining of GFAP. The sections were stained with Aves Labs anti-GFAP antibody (visualized in green) (Cat. No. GFAP) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
Aves Labs Anti-Glial Fibrillary Acidic Protein Antibody
$285
Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
PhosphoSolutions Anti-RBPMS Antibody
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Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Aves Labs Anti-Tyrosine Hydroxylase Antibody
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Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Aves Labs Anti-NFH (Neurofilament Heavy Chain) Antibody
$285
A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London. Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Antibodies Incorporated Anti-Centromere Protein Antibody
$150
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Cat. No. F-1005, 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Aves Labs Anti-Beta-Tubulin 3 Antibody
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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.IMCD3 cells transfected with Arl13b-mCherry​ and stained with Anti-mCherry Antibody (cat. MCHERRY, 1:300) and visualized​ with A594 anti-chicken secondary antibody. ​ As expected, cytoplasmic and ciliary signals are observed in transfected cells.​ Image kindly provided by Svetlana Makova, Yale University.
Aves Labs Anti-mCherry Antibody
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Immunostaining of mouse retinal ganglion cells showing specific immunolabeling of RBPMS (1:1000) in red. Photo courtesy of Allen Rodriguez, University of California, Los Angeles.Immunostaining of cat retina showing specific immunolabeling of RBPMS. Photo courtesy of Emily Brinker, Auburn University.
PhosphoSolutions Anti-RBPMS Antibody
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Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Aves Labs Anti-Iba1/AIF1 Antibody
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Western blot of mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the ~50 kDa δ-subunit of the GABAA-R.Immunostaining of a novel ChAT-δ knock down mouse brain labeling GABAA(δ)R (Cat no 868A-GDN, 1:50, green) in WT c57Bl/6 mouse brain, and confirmation of a negative signal in the ChAT-δ knock down mouse brain. Image from publication CC-BY-4.0. PMID: 37085567
PhosphoSolutions Anti-GABAA Receptor δ, N-Terminus Antibody
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Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.Immunoblot images of MAG and MBP (cat. MBP, 1:2000) in the somatosensory cortex of wildtype and GluD1 KO. Significant increase in MAG (***p < 0.0001, unpaired t-test) and MBP (*p < 0.0104, unpaired t-test) was found in GluD1 KO mice compared to wildtype. Image from publication CC-BY-4.0. PMID:37983226
Aves Labs Anti-Myelin Basic Protein Antibody
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MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)Neonatal mouse brain cortical cultures were stained with Aves Labs MAP2 (green) and GFAP (red, rabbit antibody); Blue is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
Aves Labs Anti-Microtubule-Associated Protein Antibody
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Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
PhosphoSolutions Anti-NCC (Thiazide Sensitive NaCl Cotransporter) (Thr53) Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
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Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
Aves Labs Anti-NFM (Neurofilament Medium Chain) Antibody
$285
Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.
Aves Labs Anti-Nestin Antibody
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Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
Aves Labs Anti-HA Epitope Tag Antibody
$295
In the image (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.Immunofluorescence of a section of injured (right, crushed) and normal (left) mouse sciatic nerve labeled with Anti-P-Zero Myelin Protein (1:1000, red), colabeled with Anti-Neurofilament NF-L (green), and DAPI labeling nuclei. Scale bar, 50 μm; Magnification, 20×. Image kindly provided by Prem Kumar, Department of Orthopedics and Rehabilitation, Penn State University College of Medicine, John C. Elfar lab.
Aves Labs Anti-P-Zero Myelin Protein Antibody
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Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing nuclear staining of OLIG2 positive cells within the white matter of cerebellum as expected. Inset (top left) shows higher magnification. Sections were stained with AvesLabs chicken anti-OLIG2 antibody at 1:1,000 dilution and detected with anti-chicken HRP.
Aves Labs Anti-Olig2 Antibody
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ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont
Aves Labs Anti-Choline Acetyltransferase Antibody
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A tissue section through an e18 mouse embryo showing β-galactosidase immunostaining (green staining) in the otic vesicle and adjacent otic ganglion. Hoechst dye (purple staining) shows the nuclei of cells.Basolateral amygdala from c-fos-lacZ transgenic male rats labeling LacZ (Cat no BGal, 1:1000, green). The image was kindly provided by Benedetto Sacchetti, Università di Torino.
Aves Labs Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody
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Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Aves Labs Anti-C-MYC Epitope Tag Antibody
$295
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (cat. 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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