Anti-Iba1/AIF1 Antibody

5 Citations

Our Anti-IBA1/AIF1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat IBA1/AIF1, and is affinity purified. It is great for use in ICC, WB.

Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
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Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Immunocytochemistry of iPSC-derived microglial cultures labelling Iba1 marker (Cat no IBA1-0100, 1:500, red), scale bar = 275 μm. Image kindly provided by Patricia Villegas Zafra, Achucarro Basque Center for Neuroscience.
Immunostaining of 5xFAD mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of IBA1 positive cells (cat. IBA1, 1:100; green) in the cortex. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.

Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet
Trial Size Trial Size

SKU: IBA1-0100

Volume: 500 µL
1-2 business days
Price:
$470.00

Product Details

IBA1/AIF1
AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).
Affinity purified
0.2 mg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
17 kDa
Purified recombinant human full-length IBA1/AIF1 (Uniprot P55008) expressed in E.Coli
Human, Mouse, Rat
AB_2910556
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:500-1:1000
ICC: 1:100-1:500
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
P55008

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
39514655not listedLi, X., et al. 2024. Polygenic risk for alcohol use disorder affects cellular responses to ethanol exposure in a human microglial cell model. Science Advances, eado5820.
Immunocytochemistry: Mouse
PMID Dilution Publication
39514655not listedLi, X., et al. 2024. Polygenic risk for alcohol use disorder affects cellular responses to ethanol exposure in a human microglial cell model. Science Advances, eado5820.
394099051:1000Dadario, NB, et al. 2024. Unveiling the Inflammatory Landscape of Recurrent Glioblastoma through Histological-Based Assessments. Cancers, .
Immunohistochemistry: Mouse
PMID Dilution Publication
382273431:500Savani, R., et al. 2024. Metabolic and behavioral alterations associated with viral vector-mediated toxicity in the paraventricular hypothalamic nucleus. Bioscience Reports, .
36405424not listedCarpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
35633501not listedGalloway, D.A., et al. 2022. Investigating the NLRP3 Inflammasome and its Regulator miR-223-3p in Multiple Sclerosis and Experimental Demyelination. Journal of Neurochemistry, 94-112.

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