Pyroptosis

3 products

Pyroptosis is a form of inflammatory programmed cell death, triggered by various pathological stimuli such as stroke, heart attack, cancer, and microbial infections. Pyroptosis is fundamentally distinct from other cell death pathways by its exclusive dependency on caspase-1. Because caspase-1 is a principle effector protease within the pyroptotic cell death pathway, it stands to reason that the intracellular detection of activated caspase-1 be a key indicator of a pyroptosis activity.

Our Pyroptosis/Caspase-1 Assays utilize our popular FLICA® technology to detect caspase-1 activation. These kits contain the caspase-1 inhibitor reagents, which have the preferred binding sequence for caspase-1, TyrVal-Ala-Asp (YVAD).

These kits also contain Nigericin, a potent microbial toxin derived from Streptomyces hygroscopicus, that acts as a potassium ionophore, capable of inducing a net decrease in intracellular levels of potassium which is crucial for oligomerization of the NLRP3 inflammasome and activation of caspase-1.

ICT offers pyroptosis/caspase-1 kits in both far red fluorescence and green fluorescence.

Showing 1 - 3 of 3 products

Figure 1. Signals leading to activation of the NLRP3 inflammasome.

Figure 2. C2BBe1 cells were infected with wild type Salmonella constitutively expressing mCherry (red). After 9 hours, live cells were incubated with FAM-YVAD-FMK (green cell) for 1 hour. The infected cell (red) is undergoing pyroptosis, and shows increased green fluorescence compared to uninfected cells due to caspase-1 activation. Data courtesy Knodler, Leigh A., et al. Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. PNAS. 107:41, 17733-17738 (2010).

ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Green Fluorescent

From $340.50

Figure 1. A pool of Jurkat cells was spiked with FAM-YVAD-FMK (cat. 9146) and divided into separate treatment groups. Starting with 24 hour samples and working backwards, 10 µM Nigericin was added to cells and the samples were incubated at 37°C throughout the induction process. The cells were washed and analyzed on a flow cytometer. The level of caspase-1 activity directly correlated to the period of exposure to Nigericin Data. courtesy of Mrs. Tracy Murphy, ICT (220:78).

ImmunoChemistry Technologies Nigericin

$45.25

Figure 2. THP-1 cells were treated with PMA (5 ng/mL) followed by LPS (100 ng/mL for 1 hour, lower row of images), or were untreated (upper row of images), and were then immediately stained with 660-YVAD-FMK for 1 hour at 37°C (concurrent with treatment). Cells were then stained with Hoechst 33342 and imaged. Intracellular caspase-1 activity was detected using Cy5 (Ex 620/60, Em 700/75) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Data courtesy of Mrs. Tracy Murphy (ICT 230:32).

ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Far Red Fluorescent

$372

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Pyroptosis

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