Fluorescent Enzymatic Assays

6 products

Fluorescent Enzymatic Assays are a type of biochemical assay that utilize the principal of fluorescence to measure enzymatic activity. Enzymes are biological molecules that catalyze chemical reactions in living organisms, and their activity can be quantified using fluorescent molecules as means of detection.

The advantage of using fluorescent enzymatic assays is they can provide high sensitivity, selectivity, an dynamic range for detecting and measuring enzymatic activity.

Fluorescent enzymatic assays can be designed in a variety of formats depending on the specific requirements of the experiment or application. They are often used in conjunction with other techniques and methods to provide quantitative and qualitative information about enzyme activity and function.

Our Fluorescent Assays are non-radioactive and can monitor multiple time points to follow kinetics.

Showing 1 - 6 of 6 products

Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.

Figure 2. A MPO standard curve was prepared and run, as described in the protocol, in the absence (A) or presence (B) of 20 mM catalase inhibitor. MPO standard and reaction mix were added to a 96-well black plate and incubated at room temperature in the dark for 30 minutes. The wells were read using Ex: 530nm and Em: 590nm. There is an approximately 50% reduction in signal in the presence of 20mM catalase inhibitor (note the different scales on the x-axis).

ImmunoChemistry Technologies Myeloperoxidase Detection Kit

$506

Figure 2. Bovine serum Semicarbazide-sensitive amine oxidase was serially diluted in 1X Reaction buffer. The serially diluted samples were run as described in the protocol. The samples were read after a 3 hours incubation period. Excitation: 530 nm and emission: 590 nm.

ImmunoChemistry Technologies Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection Kit

$475

ImmunoChemistry Technologies Fluorescent Monoamine Oxidase Detection Kit

$289

Figure 2. The reaction contained 20mM hydrogen peroxide (final) per well and the indicated amounts of catalase. The reaction was incubated for 30 minutes at room temperature. Reaction cocktail was added to each well and the reaction incubated for another 10 minutes in the dark at room temperature. Fluorescence was measured at excitation 530nm and emission 590nm. The graphs show the net fluorescence change: Negative Control fluorescence (no catalase) minus Sample (catalase) fluorescence.

ImmunoChemistry Technologies Fluoro Catalase Activity Detection Kit by Cell Technology

$341

ImmunoChemistry Technologies Fluoro AChE - a fluorescence assay for monitoring and detecting acetylcholinesterase activity in samples

From $486

Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Eosinophil Peroxidase Detection Kit

$475

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Fluorescent Enzymatic Assays

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