Monoclonal Antibodies

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Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
NeuroMab Anti-PSD-95 Antibody
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Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).Immunofluorescence staining of nodes of Ranvier in adult rat optic nerve with N106/36 (red) and Caspr rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
NeuroMab Anti-Ankyrin-G (Staining) Antibody
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Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
NeuroMab Anti-Arl13b Antibody
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Chemiluminescent immunoblot of endogenous LRRK2 (cat. 75-253) in cytoplasmic and membrane fractions of B16 melanocytes with Rab38 versus control siRNA knockdown. TFRC and GAPDH are used as membrane and cytoplasmic markers, respectively. Image from publication CC-BY-4.0. PMID:37625589Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).
NeuroMab Anti-LRRK2/Dardarin, C-Terminus Antibody
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Adult rat hippocampus immunohistochemistry.Adult rat whole brain immunohistochemistry.
NeuroMab Anti-Nav1.1 Na+ Channel Antibody
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Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-RGS14 Antibody
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Immunolabeling of principal neurons in the mouse neocortex specifically labeling Kv2.1 channels (1:500). The image is from a CLSM stack, pixel size is 100 nm. The image is kindly provided by Csaba Cserep, Institute of Experimental Medicine, Budapest, Hungary.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Kv2.1 K+ Channel Antibody
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Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat No. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
NeuroMab Anti-GFAP Antibody
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Immunofluorescence staining of 14 DIV cultured rat cortical neuron with N355/1. Image courtesy of Brad Elmer and Kim McAllister (UCD).Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-GluA1/GluR1 Glutamate Receptor Antibody
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Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
NeuroMab Anti-Pan-Neurofascin (Extracellular) Antibody
$374
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-CASPR/Neurexin IV Antibody
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Transfected cell immunofluorescence: COS cells expressing GFP. Blue = Hoechst nuclear stain, Green = GFP, Red = N86/8.Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/8 TC supe.
NeuroMab Anti-GFP Antibody
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Immunolabeling of postnatal 14 mouse CA3 (Axonal Initial Segment, Parvalbumin positive interneuron) labeling Kv1.2 (75-008, 1:200, yellow) Parvalbumin (magenta), and Ankyrin G (cyan). Mice were transcardially perfused with cold 4% PFA in PBS, then brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min. before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.Immunolabeling of permeabilized HEK293 cells specifically labeling intracellular Kv1.2 (Cat no 75-008, 1:200, red) and DAPI labelling DNA (blue). Images kindly provided by Ivan Talucci and Maric Hans, Rudolf Virchow Center for Integrative and Translational Bioimaging.
NeuroMab Anti-Kv1.2 K+ Channel Antibody
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Immunoblot of adult mouse membranes from wild-type (WT) and GABA-A-R-Alpha1 knockout (KO) mice probed with N95/35 (green) and anti-actin polyclonal (red). Image courtesy of Dominique Arion, Kelly Rogers and David Lewis (University of Pittsburgh).Adult rat brain membrane immunoblot.
NeuroMab Anti-GABA-A-R, Alpha1 Antibody
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Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.Representative fluorescence images of Fbxo2 (green) and NR1 (red; cat. 75-272, 1:500) fluorescence intensity in PFC slices from WT or Tau mice treated with Smyd3 inhibitor BCI121 or vehicle. Image from publication CC-BY-4.0. PMID:36609445
NeuroMab Anti-GluN1/NR1 Glutamate Receptor Antibody
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Immunolabeling of mouse hippocampus identifying GluR2 (Cat no 75-002, red). Nuclear staining done with DAPI. Image kindly provided by Huaye Zhang, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School. Electron micrograph of L21/32 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) were observed in the postsynaptic densities of dendritic spines and dendritic shafts (Den) establishing asymmetrical synapses with axon terminals (b). Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
NeuroMab Anti-GluA2/GluR2 Glutamate Receptor Antibody
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Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-GluN2B/NR2B Glutamate Receptor Antibody
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Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).Adult rat cerebellum immunohistochemistry.
NeuroMab Anti-HCN1 Antibody
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Immunofluorescence staining of adult rat cortex with N419/40 (red), K89/34 (green) and Hoechst nuclear stain (blue).Immunoblot against fractions of adult rat brain membranes (RBM) and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid probed with N419/40 (left) or K58/35 (right) TC supe.
NeuroMab Anti-Pan-Nav1 Sodium Channel Antibody
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Adult rat hippocampus immunohistochemstry.Adult rat brain membrane immunoblot.
NeuroMab Anti-GluN2A/NR2A Antibody
$374
Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.
NeuroMab Anti-Nav1.8 Na+ Channel Antibody
$374
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228HEK293T transfectants expressing SLITRK1 or its mutants were co-cultured with hippocampal neurons. Two days after co-culture, induced pre-synapses were quantified through VGAT or VGLUT1 immunostaining and GFP. Image from publication CC-BY-4.0. PMID:36085162
NeuroMab Anti-VGlut1 Antibody
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Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).Adult rat hippocampus immunofluorescence under standard protocol (left) and with antigen retrieval via pepsin pretreatment (right).
NeuroMab Anti-GluN2B/NR2B Glutamate Receptor Antibody
$374
Immunofluorescence labeling of axon initial segments in adult rat cortex with N106/65 TC supe (red) and Hoechst nuclear stain (blue).Representative images showed the high expression level of FKBP12 (green) in axonal hillock/axon initial segment (labeled by anti–ankyrin-G antibody, cat. 75-147; bright blue) under basal conditions whereas FKBP12 translocated to soma and dendrites when neurons bear tau aggregation. Image from publication CC-BY-4.0. PMID:36724228
NeuroMab Anti-Ankyrin-G (Staining) Antibody
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