Anti-Arl13b Antibody (N295B/66)

Name: Anti-Arl13b Antibody
Brand: NeuroMab
SKU: 75-287
Price: 374.0 USD
Availability: InStock

Our Anti-Arl13b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N295B/66. It is KO validated, detects human, mouse, non-human primate, rat, and zebrafish Arl13b, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.

Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.

Adult rat brain immunohistochemistry of ciliated cells surrounding the lateral ventricle (LV).

$Immunoblot versus crude membranes from adult rat brain (RBM) and pellet/lysate fractions of wild-type (WT) and Arl13b knockout (KO) mouse embryonic fibroblasts (MEF) probed with N295B/66 TC supe (left), rabbit polyclonal (middle) and N52A/42 TC supe (right). MEF cells courtesy of Laura Mariani and Tamara Caspary (Emory University SOM).$

Immunostaining of KIF24-depleted Panc1 cells in serum-starved medium treated with DMSO or 1 μM SAG for 48 h. Cells were immunostained with anti-ARL13B (green) and anti-GPR161 (red) antibodies, DNA was stained with Hoechst (blue). Image from publication CC-BY-4.0. PMID: 35803737

Semi-thin resin sections of the mouse retina were immunolabeled with affinity-purified mouse monoclonal antibody against Arl13b (NeuroMab) (H1,I1), rabbit polyclonal antibody against RIBEYE (U2656) (H2) and rabbit polyclonal antibody against PSD-95 (I2). Signals are overlaid in (H3,I3). Image from publication CC-BY-4.0. PMID: 35806143

Spinning disc confocal image identifying localization of the atypical GTPase protein ARL13B (Cat 75-287, red) in primary cilium, marked by acetylated tubulin (green), in Aquaporin 2 (magenta) positive renal collecting ducts in a 24-day old mouse. Image kindly provided by Kevin White, UTSW-Dallas.

Ciliogenesis induced mIMCD3 cells stably expressing eGFP-UBL3 WT (upper panel – arrows inside and at base of cilium) and eGFP-UBL3 C>A (middle panel – arrowheads at basal bodies/centromeres), and transiently expressing eGFP-UBL3 S>K (bottom panel – arrows inside cilium). Cilia stained with antibodies against ARL13B (cat. 75-287, 1:500; magenta). Basal bodies stained with antibodies against pericentrin (PCTN, red). Nuclei stained with DAPI (blue). Image from publication CC-BY-4.0. PMID:36672247

Representative images of immunostaining of ARL13B (cat. 75-287, 1:500; red) and K5 (green) at the base of the olfactory epithelium (OE) in WT and 123-Arl13b mice at P5. Image from publication CC-BY-4.0. PMID:36661357

Representative confocal images of WT and Dp10 mouse embryonic fibroblasts. Cells were stained with Hoechst 33342 (blue), the ciliary marker ARL13B (cat. 75-287, 1:500; green), and Actub (magenta). Image from publication CC-BY-4.0. PMID:36656118

Primary cilium formation in hTERT-RPE1 cells treated with siRNA: control (a), UBQLN4 (b), BAG6 (c), and RNF126 (d). At 72 h after transfection, hTERT-RPE1 cells were serum-starved for 24 h to trigger ciliogenesis, and the distribution of ARL13B, a marker for primary cilia, was observed (cat. 75-287 green). Control cells grew primary cilia (a), whereas those treated with knockdown of UBQLN4 (b), BAG6 (c), and RNF126 (d) failed to grow cilia. Image from publication CC-BY-4.0. PMID:37182096

Confocal images from flat mount mouse retina samples at different postnatal ages showing the peripheral or central areas of RPE. ZO-1 staining cell junctions (red), Arl13b is a ciliary marker (cat. 75-287, 1:2000; green), nuclei (blue). Magnifications of each corresponding image are shown. Image from publication CC-BY-4.0. PMID:37211572

Confocal images stained for Arl13b (cat. 75-287, 1:2000; green) and ZO-1 (red) of healthy human RPE and AMD patient. Image from publication CC-BY-4.0. PMID:37211572

ALMS1 KO1 (p.Glu2462Glyfs∗8, p.Ser2846Argfs∗10), ALMS1 KO2 (p.Asp2458Argfs∗7, p.Ser2846Lysfs∗38) and control hTERT-RPE1 cells were stained for ARL13B (cilia, cat. 75-287, 1:500; magenta) and ALMS1, acetylated tubulin or γ-tubulin (green). In control cells, ALMS1 was detected at the basal body. In the two ALMS1 KO cell lines ALMS1 was lost at the cilium. No difference was observed for ARL13B, acetylated tubulin and γ-tubulin compared to control. DAPI is marked in blue. CC-BY-4.0. PMID:38122899

Immunofluorescence staining of wild-type and Tubb4ab KO respiratory MCC at P1 showing specific staining of Tubb4 (magenta), Arl13b( cat. 75-287, 1:500; green), and FOP1 (red). Image from publication CC-BY-4.0. PMID:38031972

Representative images of MDCK WT and TTLL12 KO cells stained for actin, Arl13b (cat. 75-287, 1:500), and GT335. Image from publication CC-BY-4.0. PMID:38177908

Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.

Species Reactivity

Human, Mouse, Non-Human Primate, Rat, Zebrafish

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

Trial Size

SKU: 75-287

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

Arl13b

Target Description

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N295B/66

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Arl13b Arl2l1

Molecular Weight

50 kDa

Antigen

Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Non-Human Primate, Rat, Zebrafish

Antibody Registry ID

AB_11001586

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with Arl13a (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

UniProt Details

UniProt (Human): Q3SXY8
UniProt (Immunogen Species): Q640N2

Product Specific References for Applications and Species

Immunocytochemistry: Canine | Human | Mouse | Rat | Zebrafish
Immunohistochemistry: Human | Mouse | Non-Human PrimOJate | Rat | Xenopus | Zebrafish
Western Blot: Human | Mouse

Immunocytochemistry: Canine
PMID	Dilution	Publication
38177908	1:500	Ceglowski, J, et al. 2024. TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells. EMBO reports, 198-227.

Immunocytochemistry: Human
PMID	Dilution	Publication
39085216	1:1000	Ha, K., et al. 2024. Cilia-enriched oxysterol 7β,27-DHC is required for polycystin ion channel activation. Nature Communications, 6468.
38949024	1:500	Lo, C.H., et al. 2024. Primary cilia formation requires the Leigh syndrome-associated mitochondrial protein NDUFAF2. The Journal of Clinical Investigation, .
38767050	1:1000	Kumari, A, et al. 2024. TNF-alpha promotes cilia elongation via mixed lineage kinases signaling in mouse fibroblasts and human RPE-1 cells. Cytoskeleton, 0.
38557732	1:1000	Chen, S, et al. 2024. Base editing correction of OCRL in Lowe syndrome: ABE-mediated functional rescue in patient-derived fibroblasts. Human Molecular Genetics, 0.
38177908	1:500	Ceglowski, J, et al. 2024. TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells. EMBO reports, 198-227.
38219074	1:1000	Lin, Z., et al. 2024. Novel compound heterozygous variants in ARL13B lead to Joubert syndrome. Journal of Cellular Physiology, .
37182096	not listed	Takahashi, T., et al. 2023. Protein Quality Control Machinery Supports Primary Ciliogenesis by eliminating GDP-bound Rab8-family GTPases. iScience, 106652.
37164542	1:200	Hua, K., et al. 2023. Fixation Methods and Immunolabeling for Cilia Proteins in Ciliary and Extraciliary Locations. Methods in Cell Biology, 43-57.
36684387	1:2000	Ning, K., et al. 2023. Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids. Stem Cells International, 6494486.
36672247	1:500	Faber, S., et al. 2023. PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia. Cells, 312.
36652335	not listed	Palicharla, V.R., et al. 2023. Interactions Between TULP3 Tubby Domain and ARL13B Amphipathic Helix Promote Lipidated Protein Transport to Cilia. Molecular Biology of the Cell, mbcE22100473.
36606424	1:100	Odabasi, E., et al. 2023. CCDC66 Regulates Primary Cilium Length and Signaling via Interactions with Transition Zone and Axonemal Proteins. Journal of Cell Science, jcs260327.
35803737	1:1000	Mashima, Y., et al. 2022. KIF24 Depletion Induces Clustering of Supernumerary Centrosomes in PDAC Cells. Life Science Alliance, .
35289477	1:100	Jabali, A., et al. 2022. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation. EMBO reports, e54027.
35253837	1:250	Afanasyeva, T.A.V., et al. 2022. PCARE requires coiled coil, RP62 kinase binding, and EVH1 domain binding motifs for ciliary expansion. Human molecular genetics, .
34678163	1:300	Rivera-Molina, F.E., et al. 2021. Exocyst complex mediates recycling of internal cilia. Current biology : CB, 5580-5589.e5.
33727250	1:2000	Dhekne, H.S., et al. 2021. LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade. Life Science Alliance, e202101050.
33206585	not listed	Failler, M., et al. 2021. Whole-genome screen identifies diverse pathways that negatively regulate ciliogenesis. Mol Biol Cell., 169-185.
33251215	1:1000	Kobayashi, T., et al. 2020. CEP164 Deficiency Causes Hyperproliferation of Pancreatic Cancer Cells. Frontiers in Cell and Developmental Biology, 587691.
32555591	1:500	Aydin, Ö.Z., et al. 2020. Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium. PLoS Biology, e3000679.
31388414	1:300	Lauring, M.C., et al. 2019. New software for automated cilia detection in cells (ACDC). Cilia, 1.
31023719	1:1000	Odabasi, E., et al. 2019. Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation. EMBO Reports, e47723.
30683896	1:300	Insinna, C., et al. 2019. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nature Communications, 428.
30622176	1:500	Zhang, Q., et al. 2019. Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP. PNAS: USA, 1353-1360.
30398148	1:1000	Dhekne, H.S., et al. 2018. A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. Elife, e40202.
30377171	1:100	Kulkarni, S.S., et al. 2018. WDR5 regulates left-right patterning via chromatin-dependent and -independent functions. Development, dev159889.
30131441	1:50 (supe)	Kurtulmus, B., et al. 2018. LRRC45 contributes to early steps of axoneme extension. Journal of Cellular Science, jcs223594.
30100262	not listed	Galati, D.F., et al. 2018. Trisomy 21 Represses Cilia Formation and Function. Developmental Cell, 641-650.
29615496	not listed	Beyer, T., et al. 2018. CRISPR/Cas9-mediated Genomic Editing of Cluap1/IFT38 Reveals a New Role in Actin Arrangement. Molecular and Cellular Proteomics, 1285-1294.
29592971	not listed	Revenkova, E., et al. 2018. The Joubert syndrome protein ARL13B binds tubulin to maintain uniform distribution of proteins along the ciliary membrane. Journal of Cellular Science, jcs212324.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37819632	1:200	Dekky, B, et al. 2023. ADAMTS12 is a stromal modulator in chronic liver disease. FASEB Journal, e23237.
37164542	1:200	Hua, K., et al. 2023. Fixation Methods and Immunolabeling for Cilia Proteins in Ciliary and Extraciliary Locations. Methods in Cell Biology, 43-57.
36967142	1:200	Dupuy, A.M.M., et al. 2023. Using Mammary Organoids to Study Cilia. Methods in Cell Biology, 221-233.
36546837	1:500	Tholen, L.E., et al. 2022. Transcription factor HNF1β Controls a Transcriptional Network Regulating Kidney Cell Structure and Tight Junction Integrity. American Journal of Physiology. Renal Physiology, .
36322399	not listed	Nguyen, A., et al. 2022. TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins. Molecular Biology of the Cell, .
35899529	1:1000	Lv, B., et al. 2022. Arih2 Regulates Hedgehog Signaling Through Smoothened Ubiquitination and ER-Associated Degradation. Journal of Cell Science, .
35322202	1:500	Li, Q., et al. 2022. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell death and differentiation, .
34914639	1:100	Zhang, W.W., et al. 2022. Peripheral ablation of type Ⅲ adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight, e153191.
34783461	1:1000	Yang, D., et al. 2022. Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy. Advanced Science, e2102568.
34463328	1:500	Mecklenburg, N., et al. 2021. Identification of disease-relevant modulators of the SHH pathway in the developing brain. Development, .
34346313	1:500	Hwang, S., et al. 2021. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner. Elife, e67121.
34055805	1:200	Paulson, D., et al. 2021. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation. Frontiers in Cell and Developmental Biology, 672625.
33989527	not listed	Ganga, A.K., et al. 2021. Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Current biology, 2895-2905.
33989524	not listed	Stuck, M.W., et al. 2021. Rab34 is necessary for early stages of intracellular ciliogenesis. Current biology, 2887-2894.
33741721	1:1000	Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
33727250	1:2000	Dhekne, H.S., et al. 2021. LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade. . Life science alliance, 5.
33653948	1:2000	Sobu, Y., et al. 2021. Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins. PNAS: USA, e2005894118.
33372037	1:1000	Barbeito, P., et al. 2020. HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails. Life Science Alliance, e202000746.
33350496	1:500	La Sala, G., et al. 2020. Gpr37l1/prosaposin receptor regulates Ptch1 trafficking, Shh production, and cell proliferation in cerebellar primary astrocytes. Journal of Neuroscience Research, .
33082319	1:2000	Wei, T., et al. 2020. Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy. Nature Communications, 5312.
32531277	1:1000	Ho, E.K., et al. 2020. Transient Primary Cilia Mediate Robust Hedgehog Pathway-Dependent Cell Cycle Control. Current Biology, 2829-2835.
32494665	not listed	Prosseda, P.P., et al. 2020. Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Science Advances, eaay8699.
32435793	1:1000	Desai, P.B., et al. 2020. Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. Journal of Cellular Biology, e201912104.
32129762	1:1000	Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
31761534	1:1000	Hilgendorf, K.I., et al. 2019. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis. Cell, 1289-1305.
31391239	1:2000	Muñoz-Estrada, J., et al. 2019. Ahi1 promotes Arl13b ciliary recruitment, regulates Arl13b stability and is required for normal cell migration. Journal of Cellular Science, jcs230680.
31390572	1:50	Uzquiano, A., et al. 2019. Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia. Cell Reports, 1596-1611.
31388414	1:300	Lauring, M.C., et al. 2019. New software for automated cilia detection in cells (ACDC). Cilia, 1.
31249512	1:300	Bansal, R., et al. 2019. Hedgehog Pathway Activation Alters Ciliary Signaling in Primary Hypothalamic Cultures. Frontiers in Cellular Neuroscience, 266.
31186137	not listed	Wang, B., et al. 2019. Cilia function is associated with axon initial segment morphology. Biochemical and Biophysical Research Communications, 15-21.
31068677	1:500	Wildung, M., et al. 2019. Transcription factor TAp73 and microRNA-449 complement each other to support multiciliogenesis. Cell Death and Differentiation, 2740-2757.
30905605	1:200	Engelke, M.F., et al. 2019. Acute Inhibition of Heterotrimeric Kinesin-2 Function Reveals Mechanisms of Intraflagellar Transport in Mammalian Cilia. Current Biology, 1137-1148.
30478281	not listed	Kilander, M.B., et al. 2018. A rare human CEP290 variant disrupts the molecular integrity of the primary cilium and impairs Sonic Hedgehog machinery. Scientific Reports, 17335.
30463852	1:500	Hsiao, C.J., et al. 2018. Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia. Journal of Cellular Science, jcs221218.
30410731	1:1000	Hoang-Minh, L.B., et al. 2018. Glioma cell proliferation is enhanced in the presence of tumor-derived cilia vesicles. Cilia, 6.
30398148	1:1000	Dhekne, H.S., et al. 2018. A pathway for Parkinson’s Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. Elife, 7, p.e40202.. Elife, e40202.
29945904	1:2000	Drummond, M.L., et al. 2018. Actin polymerization controls cilia-mediated signaling. Journal of Cellular Biology, 3255-3266.
29678909	1:1000	Lesiak, A.J., et al. 2018. Restoration of Physiological Expression of 5-HT 6 Receptor into the Primary Cilia of Null Mutant Neurons Lengthens Both Primary Cilia and Dendrites. Molecular Pharmacology, 731-742.
29571613	1:500	Gigante, E.D., et al. 2018. Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response. Developmental Biology, 152-162.
29540531	1:1000	Prinz, E., et al. 2018. Intraflagellar transport is deeply integrated in hedgehog signaling. Molecular Biology of the Cell, 1178-1189.
28931427	not listed	Hu, L., et al. 2017. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Research and Therapy, 76.
28611083	1:400	Hassounah, N.B., et al. 2017. Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer. Molecular Cancer Research, 1421-1430.
28530676	1:300	Lu, H., et al. 2017. Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. Nature Genetics, 1025-1034.
28459465	not listed	Yang, N., et al. 2017. INTU is essential for oncogenic Hh signaling through regulating primary cilia formation in basal cell carcinoma. Oncogene, 4997-5005.
28410364	not listed	Pearring, J.N., et al. 2017. Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genetics, e1006740.
28352462	1:200	Hua, K., et al. 2017. Fixation methods can differentially affect ciliary protein immunolabeling.. Cilia., 5.
28154160	not listed	Badgandi, H.B., et al. 2017. Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.. J Cell Biol., 743-760.

Immunocytochemistry: Rat
PMID	Dilution	Publication
34658337	1:1000	Khan, S.S., et al. 2021. Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain. Elife, e67900.
33650969	1:1000	Tereshko, L., et al. 2021. Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons. Elife, e65427.

Immunocytochemistry: Zebrafish
PMID	Dilution	Publication
33932338	1:1000	Truong, M.E., et al. 2021. Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Cell, 2911-2926.

Immunohistochemistry: Human
PMID	Dilution	Publication
37211572	1:2000	Ning, K, et al. 2023. Cilia-associated wound repair mediated by IFT88 in retinal pigment epithelium. Scientific reports, 8205.
36580912	not listed	Wu, C.T., et al. 2022. SARS-CoV-2 Replication in Airway Epithelia Requires Motile Cilia and Microvillar Reprogramming. Cell, .
34496239	1:500	Goranci-Buzhala, G., et al. 2021. Cilium induction triggers differentiation of glioma stem cells. Cell Reports, 109656.
33619909	1:400	Dang, L.T., et al. 2021. STRADA-mutant human cortical organoids model megalencephaly and exhibit delayed neuronal differentiation. Developmental Neurobiology, 696-709.
33306870	not listed	Ning, K., et al. 2020. Defective INPP5E distribution in NPHP1-related Senior-Loken syndrome. Molecular Genetics and Genomic Medicine, e1566.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37858465	1:500	Hoi, KK, et al. 2023. Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. Cell Reports, 113272.
37211572	1:2000	Ning, K, et al. 2023. Cilia-associated Wound Repair Mediated by IFT88 in Retinal Pigment Epithelium. Scientific Reports, 8205.
36693320	1:1000	Zhang, K., et al. 2023. Primary Cilia are WNT-transducing Organelles Whose Biogenesis is Controlled by a WNT-PP1 Axis. Developmental Cell, 139-154.
36661357	1:500	Habif, J.C., et al. 2023. The Role of a Ciliary GTPase in the Regulation of Neuronal Maturation of Olfactory Sensory Neurons. Development, .
36656118	1:500	Jewett, C.E., et al. 2023. Trisomy 21 Induces Pericentrosomal Crowding Delaying Primary Ciliogenesis and Mouse Cerebellar Development. eLife, .
36496200	1:1000	Dupuy, V., et al. 2023. Spatiotemporal Dynamics of 5-HT6 Receptor Ciliary Localization During Mouse Brain Development. Neurobiology of Disease, 105949.
34057457	not listed	Duclos, M., et al. 2021. Three-Dimensional Imaging Of Organoids To Study Primary Ciliogenesis During Ex Vivo Organogenesis. Journal Of Visualized Experiments, .
35806143	1:200	Suiwal, S., et al. 2022. Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. International Journal of Molecular Sciences, 7135.
35322202	1:500	Li, Q., et al. 2022. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell death and differentiation, .
34705506	1:100	Wilson, M.M., et al. 2021. An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. Science Advances, eabf6063.
34610637	1:500	Lo, H.F., et al. 2021. Δ9-Tetrahydrocannabinol inhibits Hedgehog-dependent patterning during development. Development, .
34492310	1:500	Boschen, K.E., et al. 2021. Prenatal alcohol exposure disrupts Sonic hedgehog pathway and primary cilia genes in the mouse neural tube. Reproductive Toxicology, 136-147.
34366878	not listed	Ningappa, M., et al. 2021. Mechanisms of Impaired Lung Development and Ciliation in Mannosidase-1-Alpha-2 ( Man1a2) Mutants. Frontiers in Physiology, 658518.
34132778	1:500	Suciu, S.K., et al. 2021. Smoothened and ARL13B are critical in mouse for superior cerebellar peduncle targeting. Genetics, iyab084.
34057457	not listed	Duclos, M., et al. 2021. Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis. Journal of Visualized Experiments, .
34055805	1:4000	Paulson, D., et al. 2021. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation. Frontiers in Cell and Developmental Biology, 672625.
33741721	1:1000	Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
33711342	1:200	Sharif, A.S., et al. 2021. Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis. Journal of Biological Chemistry, 100529.
33175436	1:500	Ramos, C., et al. 2021. Neuron-specific cilia loss differentially alters locomotor responses to amphetamine in mice. Journal of Neuroscience Research, 827-842.
33103737	1:500	Diaz, J., et al. 2020. YIF1B mutations cause a post-natal neurodevelopmental syndrome associated with Golgi and primary cilium alterations. Brain, 2911-2928.
33082319	1:2000	Wei, T., et al. 2020. Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy. Nature Communications, 5312.
33046507	1:5 (supe)	Casoni, F., et al. 2020. ZFP423 regulates early patterning and multiciliogenesis in the hindbrain choroid plexus. Development, dev190173.
32938678	not listed	Dias, J.M., et al. 2020. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells. Science Advances, eaba8196.
32917588	1:1000	Stoufflet, J., et al. 2020. Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration. Science Advances, eaba3992.
32840212	1:1500	, et al. 2020. A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development. Elife, e58162.
32702291	1:500	Somatilaka, B.N., et al. 2020. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Developmental Cell, 710-726.
32169553	1:50	Fiore, L., et al. 2020. Optic vesicle morphogenesis requires primary cilia. Developmental Biology, 119-128.
32129762	1:1000	Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
32122360	1:250	Sterpka, A., et al. 2020. Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults. Molecular Brain, 28.
31964774	1:2000	Constable, S., et al. 2020. The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling. Development, dev183301.
31934864	1:500	Bowie, E., et al. 2020. TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife, e51166.
31887266	not listed	Shylo, N.A., et al. 2020. Loss of ciliary transition zone protein TMEM107 leads to heterotaxy in mice. Developmental Biology, 187-199.
31690747	1:500	Fish, E.W., et al. 2019. Cannabinoids Exacerbate Alcohol Teratogenesis by a CB1-Hedgehog Interaction. Scientific Reports, 16057.
31685650	1:1000	Matsumoto, M., et al. 2019. Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain. Journal of Neuroscience, 9967-9988.
31321879	1:200	Freeman, S., et al. 2019. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Reports, e47097.
30904180	1:500	Gigante, E.D., et al. 2019. Corrigendum to "Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response" [Develop. Biol. 437 (2018) 152-162]. Developmental Biology, 63.
30799240	1:500	Legué, E., et al. 2019. Tulp3 Is a Ciliary Trafficking Gene that Regulates Polycystic Kidney Disease. Current Biology, 803-812.
30799239	1:500	Hwang, S.H., et al. 2019. Tulp3 Regulates Renal Cystogenesis by Trafficking of Cystoproteins to Cilia. Current Biology, 790-802.
30695697	not listed	Chang, C.H., et al. 2019. Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. Developmental Cell, 184-199.
30692221	1:1500	Andreu-Cervera, A., et al. 2019. The Ciliopathy Gene Ftm/Rpgrip1l Controls Mouse Forebrain Patterning via Region-Specific Modulation of Hedgehog/Gli Signaling. Journal of Neuroscience, 2398-2415.
29342244	1:500	Chen, J.L., et al. 2019. Gli2 Rescues Delays in Brain Development Induced by Kif3a Dysfunction. Cerebral Cortex, 751-764.
30157434	not listed	Haag, N., et al. 2018. The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Reports, 2418-2431.
29626631	not listed	Desai, P.B., et al. 2018. Ift25 is not a cystic kidney disease gene but is required for early steps of kidney development. Mechanisms of Development, 44851.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
31685650	1:1000	Matsumoto, M., et al. 2019. Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain. Journal of Neuroscience, 9967-9988.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31825820	1:2000	Ferent, J., et al. 2019. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance. Cell Reports, 3356-3366.

Immunohistochemistry: Xenopus
PMID	Dilution	Publication
37172641	1:100	Deniz, E, et al. 2023. CFAP45, a Heterotaxy and Congenital Heart Disease Gene, Affects Cilia Stability. Developmental Biology, 75-88.

Immunohistochemistry: Zebrafish
PMID	Dilution	Publication
31503551	1:150	Arribat, Y., et al. 2019. Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy. Journal of Clinical Investigation, 5312-5326.
30683896	1:300	Insinna, C., et al. 2019. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nature Communications, 428.

Western Blot: Human
PMID	Dilution	Publication
35147977	1:1000	Huang, M., et al. 2022. Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly. Journal of cellular physiology, .
31023719	1:500	Odabasi, E., et al. 2019. Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation. EMBO Reports, e47723.

Western Blot: Mouse
PMID	Dilution	Publication
36661357	1:500	Habif, J.C., et al. 2023. The Role of a Ciliary GTPase in the Regulation of Neuronal Maturation of Olfactory Sensory Neurons. Development, .
35259752	1:1000	Hamada, N., et al. 2022. Expression Analyses of Cep152, a Responsible Gene Product for Autosomal Recessive Primary Microcephaly, During Mouse Brain Development. Developmental Neuroscience, 162-170.
33741721	1:1000	Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
32129762	1:1000	Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
30799240	not listed	Legué, E., et al. 2019. Tulp3 Is a Ciliary Trafficking Gene that Regulates Polycystic Kidney Disease. Current Biology, 803-812.
28931427	not listed	Hu, L., et al. 2017. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Research and Therapy, 76.
28576874	not listed	Monis, W.J., et al. 2017. BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. Journal of Cell Biology, 2131-2150.

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Anti-Arl13b Antibody (N295B/66)

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