Cytotoxicity

7 products

Showing 1 - 7 of 7 products
View
Figure 1. Quantification of cell deathFigure 2. Assay procedure
ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test
From $173
Choose options
Figure 1. K562 cells were stained with CFSE analog. Lymphocytes were added at: effector (E)/ target (T) ratios of 25:1, 50:1, and 100:1. Following incubation, 7AAD (live/dead) stain was added and cells analyzed via flow cytometry. % Cytotoxicity can be determined by an increase of target cells (lower right quadrant) moving into upper right quadrant and calculated by (R1/(R1+R2))*100.Figure 2. A linear increase indicates the assay is optimized and an E/T ratio will be optimal for the experiments.
ImmunoChemistry Technologies Flow Cytometry Cytotoxicity Assay
$310
5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2 . The ADCC reaction was further incubated for 3.5 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay. 3.5hour timepoint has been optimized with the 10:1 E:T ratio.
ImmunoChemistry Technologies aCella - Tox - an assay designed to measure cell cytoxicity
From $883
Choose options
Figure 1. Quantification of 4 populations in each sampleFigure 2. Assay procedure
ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Total Cytotoxicity Test
From $373
Choose options
Figure 1. Fluorescence spectraFigure 2. Jurkat cells were exposed to 3% formaldehyde for 30 minutes. Following the formaldhehyde treatment, cells were stained with Green Live/ Dead stain and then imaged with a Nikon E800 microscope (DIC overlay shown). Cells with compromised membranes stained green, while cells with intact membranes excluded the Green Live/Dead stain and remained unstained. Data courtesy of Mrs. Tracy Murphy, ICT.
ImmunoChemistry Technologies Green Live/Dead Stain
$102.50
Figure 1. Live cells with green fluorescence (right side of histogram) can be distinguished from dead cells (unstained, left side of histogram). Forward and side scatter graphs are also shown. Jurkat cells were stained with 1 µM Calcein AM and analyzed in a flow cytometer with a FL-1 99% attenuation filter. 93.0% of the live cells stained positive with Calcein AM (A, right), while 0.0% of ethanol-killed cells stained positive (B, right). Data courtesy of Dr. Kristi Strandberg, ICT 226:87-92.Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of ethanol-treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.
ImmunoChemistry Technologies Advanced Calcein AM Cell Viability Kit
$290
Figure 1. Live cells exhibiting green fluorescence (right of histogram) are easily distinguished from dead cells (unstained, left of histogram). Jurkat cells were either mock-treated (A) or killed by exposure to 90% ethanol (B). Cells were stained with Calcein AM, then analyzed in a flow cytometer with a FL-1 99% attenuation filter. Most (93.0%) live cells stained positive with Calcein AM (A, right), while 0.0% of the dead cells stained positive (B, right). Data courtesy of Dr. K. Strandberg.Figure 2. Mock-treated Jurkat suspension cells were stained with Calcein AM to detect live cells. The majority of the cells imaged were considered to be live and healthy. Panel A reveals green fluorescence-stained live cells. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology. Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 226:95).
ImmunoChemistry Technologies Basic Calcein AM Cell Viability Kit
$234

Recently Viewed