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PhosphoSolutions 10X Lysis Buffer

$120

Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).

Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).

ImmunoChemistry Technologies 7-AAD Red Fluorescent Live/Dead Stain

$177.20

ImmunoChemistry Technologies 96-Well Plate Template Post-It Notes

$8

ImmunoChemistry Technologies ABTS 1-Component HRP Microwell Substrate (SUBA)

$45.40

5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.

ImmunoChemistry Technologies aCella - Tox - an assay designed to measure cell cytoxicity

From $910

Figure 1. Normal Jurkat cells stained with Acridine Orange (AO) show orange lysosomal staining. Cells were stained with 5 µM AO in PBS for 60 minutes at 37°C. Photomicrographs were taken using a Nikon Eclipse E800 photomicroscope using a 460-500 nm excitation filter and a 505-560 nm emission / barrier filter set at 300X. Photo B shows the corresponding DIC image of the cells in A (AO appears faintly). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Institute, New York, NY).

Figure 2. MCF-7 cells were treated with camptothecin to induce apoptosis (cat. 6210), stained with AO in PBS, washed, and then photographed with an epifluorescence microscope at 40X using either a blue light excitation (492 nm) with a 540- 550 nm emission filter (A, lysosomes appear yellowish green), or green light excitation (540 nm) with a long pass >640 nm barrier filter (B, lysosomes appear red). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Center Institute, New York, NY).

ImmunoChemistry Technologies Acridine Orange Fluorescent Staining Solution

$23.40

Antibodies Incorporated Ad 26 Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 3 Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 35 Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 37 Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 4 Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 5 ARM (Adenovirus Reference Material) Wild-Type Adenovirus Particle

$670

Antibodies Incorporated Ad 8 Wild-Type Adenovirus Particle

From $300

Antibodies Incorporated Ad CMV AlkPhos Adenovirus

$670

Antibodies Incorporated Ad CMV CD54 Adenovirus

$670

Antibodies Incorporated Ad CMV Cre Adenovirus

$670

Antibodies Incorporated Ad CMV CXCL10 Adenovirus

$670

Antibodies Incorporated Ad CMV Cyto LacZ Adenovirus

$670

Antibodies Incorporated Ad CMV eGFP Adenovirus

$670

Antibodies Incorporated Ad CMV eGFP F35 Adenovirus

$670

Antibodies Incorporated Ad CMV GM-CSF2 Adenovirus

$670

Antibodies Incorporated Ad CMV hTSHR 289 Adenovirus

$670

Antibodies Incorporated Ad CMV hVEGF 165 Adenovirus

$670

Antibodies Incorporated Ad CMV IL-12 Adenovirus

$670

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