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PhosphoSolutions 10X Lysis Buffer

$115

Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).

Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).

ImmunoChemistry Technologies 7-AAD Red Fluorescent Live/Dead Stain

$172

ImmunoChemistry Technologies 96-Well Plate Template Post-It Notes

$7.80

ImmunoChemistry Technologies ABTS 1-Component HRP Microwell Substrate (SUBA)

$44

5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.

ImmunoChemistry Technologies aCella - Tox - an assay designed to measure cell cytoxicity

From $883

Figure 1. Normal Jurkat cells stained with Acridine Orange (AO) show orange lysosomal staining. Cells were stained with 5 µM AO in PBS for 60 minutes at 37°C. Photomicrographs were taken using a Nikon Eclipse E800 photomicroscope using a 460-500 nm excitation filter and a 505-560 nm emission / barrier filter set at 300X. Photo B shows the corresponding DIC image of the cells in A (AO appears faintly). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Institute, New York, NY).

Figure 2. MCF-7 cells were treated with camptothecin to induce apoptosis (cat. 6210), stained with AO in PBS, washed, and then photographed with an epifluorescence microscope at 40X using either a blue light excitation (492 nm) with a 540- 550 nm emission filter (A, lysosomes appear yellowish green), or green light excitation (540 nm) with a long pass >640 nm barrier filter (B, lysosomes appear red). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Center Institute, New York, NY).

ImmunoChemistry Technologies Acridine Orange Fluorescent Staining Solution

$22.75

Figure 1. Live cells with green fluorescence (right side of histogram) can be distinguished from dead cells (unstained, left side of histogram). Forward and side scatter graphs are also shown. Jurkat cells were stained with 1 µM Calcein AM and analyzed in a flow cytometer with a FL-1 99% attenuation filter. 93.0% of the live cells stained positive with Calcein AM (A, right), while 0.0% of ethanol-killed cells stained positive (B, right). Data courtesy of Dr. Kristi Strandberg, ICT 226:87-92.

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of ethanol-treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Advanced Calcein AM Cell Viability Kit

$290

ImmunoChemistry Technologies AEC Peroxidase Chromogen Substrate Kit

$113.75

ImmunoChemistry Technologies Alkaline Phosphatase Conjugate Stabilizer, 1X

From $27.40

ImmunoChemistry Technologies Alkaline Phosphatase Enhancer

$113.75

ImmunoChemistry Technologies Alternative Block ELISA Blocking

From $52.25

Antibodies Incorporated ANA Kit (10 x 12-well slides)

$355

Antibodies Incorporated ANA Kit (20 x 12-well slides)

$600

Antibodies Incorporated ANA Negative Control

$55

Antibodies Incorporated ANA Positive Control (Homogeneous)

$55

Antibodies Incorporated ANA Speckled Positive Control

$65

Antibodies Incorporated ANA Substrate slides (20 x 12 well)

$430

ImmunoChemistry Technologies Annexin V-FITC Apoptosis Assay Kit

$267

Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58(cat. p1433-58, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).

PhosphoSolutions Anti-14-3-3 (Ser58) Antibody

From $155

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein.

PhosphoSolutions Anti-14-3-3 Protein Antibody

From $155

Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).

NeuroMab Anti-5-Hydroxymethylcytidine Antibody

$319

Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

PhosphoSolutions Anti-5-Lipoxygenase (Ser523) Antibody

From $155

Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.

Immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.

NeuroMab Anti-6xHis Antibody

$319

NeuroMab Anti-6xHis Antibody FL490 Conjugate

$460

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