Primary Antibodies

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HeLa cells were subjected to immunofluorescent staining using chicken anti-TOMM20 antibody (visualized in green) and Antibodies Inc mouse anti-Mortalin antibody (75-127) (visualized in red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (AR-6501). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mortalin antibody specifically in the cell mitochondria.Western blotting of various cell lysates with chicken anti-TOMM20 antibody (0.2 µg/ml)(1:1000) and detected with anti-chicken HRP. Chicken anti-TOMM20 recognizes endogenous TOMM20 in all the cell lysates at ~16 kDa.
Aves Labs Anti-TOMM20 Antibody
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Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat no. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.​Western blotting of MCF7 cell lysates with  chicken anti-HB9 (MNX1) antibody at various concentrations and detected with anti-chicken HRP. Chicken anti-HB9/MNX1 recognizes endogenous MNX1 in MCF7 cell lysates at ~50 kDa.
Aves Labs Anti-MNX1/H9B Antibody
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Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).
Immunohistochemistry of 10 micron cryostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion showing specific staining of Notch 3.
Western Blot of 10 ug of normal brain lysate showing specific immunolabeling of MBP.
Western Blot of 10 ug of normal human brain lysate showing specific immunolabeling of CNPase.
 Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ
Western Blot of 3 ug of RSV virions (subgroup A-Long strain) showing specific immunolabeling of Respiratory Syncytial Virus.  The antibody detects the following RSV proteins: Glycoprotein (G) ~90 kDa, Fusion (F) protein ~55 kDa, and nucleocapsid (N) protein ~46 kDa.  Overexposure of the blot can result in detection of M2 protein (~22 kDA-not shown).
Western Blot of 3 ug of intact A/California/14/2009 H1N1 virions showing specific immunolabeling of Influenza A H1N1.  This antibody detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).
Western Blot of 10 ug of human brain lysate (lane 1), rat brain lysate (lane 2) and mouse brain lysate (lane 3) showing specific immunolabeling of MOR-1.

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