Anti-VGlut1 Recombinant Chicken Chimeric mAb (N28/9)
Our chicken Anti-VGlut1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N28/9. It detects fish, human, mouse, and rat VGlut1, and is purified by affinity chromatography. It is great for use in Flow, IHC, ICC, WB.



Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut1. The sections were stained with anti-VGlut1 recombinant chicken monoclonal antibody (green) (Cat No. 78-066) at 1:100 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut1 specifically stains the axonal synapses in the granular layer of the cerebellum. Magnification 100X.
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Fish, Human, Mouse, Rat
ELISA, ICC, IHC, WB
Chicken

SKU: 78-066
Ships: 1-2 business days
Product Details
VGlut1
Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.
Purified by affinity chromatography
1 mg/mL
Recombinant
N28/9
IgY
ELISA, ICC, IHC, WB
Chicken
Slc17a7 Bnpi Vglut1
52 kDa
Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli
Fish, Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N28/9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N28/9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
Unconjugated
Does not cross-react with VGlut2 or VGlut3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Vesicular glutamate transporter 1 (VGluT1) (Brain-specific Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 7)