ICT’s Substrates enhance the sensitivity of your ELISA, reduce background noise, provide linearity for enhanced reproducibility, and are non-toxic and environmentally friendly.
ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors.
Our STOP Solutions stop the color development, standardize your readings and reduce variation for enhanced reproducibility. We offer stop solutions for HRP and alkaline phosphatase reactions.