Anti-VGlut2 Recombinant Chicken Chimeric mAb (N29/29)
Our chicken Anti-VGlut2 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N29/29. It detects electric ray, human, mouse and rat VGlut2, and is purified by affinity chromatography. It is great for use in ELISA, IHC, ICC, WB.



Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut2 with anti-VGlut2 recombinant chicken monoclonal antibody (green) (Cat. 78-067) at 1:100 dilution. The sections were mounted with FluoroshieldTM with DAPI and DABCO mounting medium (Cat AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut2 specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 100X.
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Chicken, Electric Ray, Human, Mouse, Rat
ELISA, ICC, IHC, WB
Chicken

SKU: 78-067
Ships: 1-2 business days
Product Details
VGlut2
Vesicular glutamate transporter 2 (VGlut2) is encoded by the gene Slc17a6 and belongs to the vesicular glutamate transporter family. Vglut2 is expressed in the thalamus, midbrain, brainstem, and spinal cord at synaptic vesicles. It functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut2 has been implicate in include Gnathodiaphyseal Dysplasia and tendinosis.
Purified by affinity chromatography
1 mg/mL
Recombinant
N29/29
IgY
ELISA, ICC, IHC, WB
Chicken
Slc17a6 Dnpi Vglut2
60 kDa
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession number Q9JI12) produced recombinantly in E. Coli
Chicken, Electric Ray, Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:400
IHC: 1:1000
ICC: 1:1000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Does not cross-react with VGlut1 or VGlut3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Vesicular glutamate transporter 2 (VGluT2) (Differentiation-associated BNPI) (Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 6)