Anti-PSD-95 Recombinant Chicken Chimeric mAb (K28/43)
Our chicken Anti-PSD 95 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by affinity chromatography. It is great for use in IHC, ICC, IP, WB.



Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of PSD-95. The sections were stained with anti-PSD-95 recombinant chicken monoclonal antibody (green) (Cat No. 78-028) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum. Magnification 100X.
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Chicken, Human, Mouse, Non-Human Primate, Rat
ELISA, ICC, IHC, IP, WB
Chicken


SKU: 78-028
Ships: 1-2 business days
Product Details
PSD 95
PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy
Purified by affinity chromatography
1 mg/mL
Recombinant
K28/43
IgY
ELISA, ICC, IHC, IP, WB
Chicken
DLG4 PSD95
95-110 kDa (varies with cell background due to phosphorylation)
Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli
Chicken, Human, Mouse, Non-Human Primate, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:500
ICC: 1:500
IHC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)