Our Anti-NCAM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NCAM1, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.
Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of NCAM1. The sections were stained with anti-NCAM1 chicken polyclonal antibody (visualized in green) at 1 µg/mL (1:200) dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat no. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.
Neural cell adhesion molecule 1 (NCAM1), also known as CD56, is a glycoprotein first characterized for its role in neural development and cell-cell adhesion. It is widely expressed in neurons, glial cells, skeletal muscle, and immune cells, including natural killer (NK) cells. NCAM1 plays a crucial role in neuron-neuron adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. It interacts with fibroblast growth factor receptors (FGFRs) to influence intracellular signaling pathways. NCAM1 has been implicated in neurological disorders such as schizophrenia and bipolar disorder, as well as in certain immune-related diseases. Due to its role in neural plasticity and immune function, NCAM1 remains a key target in research on brain repair, psychiatric disorders, and immunotherapy.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
NCAM1
Calculated - 95 kDa; Observed - 120 - 180 kDa
Human NCAM1 1-718 (Extracellular region)
Human, Mouse, Rat
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
