Our Anti-SQSTM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat SQSTM1, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.
Immunofluorescent staining of mock- or 50 µM chloroquine-treated HeLa cells using 2 µg/mL chicken anti-SQSTM1 (p62) antibody (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). Anti-SQSTM1 (p62) specifically stains the autophagosomes in HeLa cells. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.
Sequestosome 1 (SQSTM1), also known as p62, is a multifunctional adaptor protein involved in autophagy, cell signaling, and protein degradation. It plays a key role in selectively targeting ubiquitinated proteins for degradation via the autophagy-lysosome pathway and is also involved in NF-κB signaling, oxidative stress responses, and bone metabolism. Mutations in SQSTM1 are strongly associated with Paget’s disease of bone and have been implicated in neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and certain cancers. Due to its role in cellular homeostasis, SQSTM1 remains a significant focus in research on autophagy-related diseases and targeted therapies.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
SQSTM1
Calculated - 48 kDa; Observed - 62 kDa
Human SQSTM1 full sequence
Human, Mouse, Rat
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe HeLa cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
