Anti-Bassoon Recombinant Chicken Chimeric mAb (L124/59)
Our chicken Anti-Bassoon recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L124/59. It detects mouse and rat Bassoon, and is purified by affinity chromatography. It is great for use in AT, ELISA, IHC, ICC, WB.


Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with anti-Bassoon recombinant chicken monoclonal antibody (green) (Cat No. 78-491) at 1:100 dilution. The sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Bassoon stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.
Roll over image to zoom in
Click on image to zoom
/
SKU: 78-491
Ships: 1-2 business days
Product Details
Bassoon
Bassoon is a large (420 kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).
Purified by affinity chromatography
1 mg/mL
Recombinant
L124/59
IgY
AT, ELISA, ICC, IHC, WB
Chicken
Bsn
400 kDa for full-length protein, many smaller bands possible depending on sample used
Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli
Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
IHC: 1:500
ICC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Protein bassoon