Anti-GAD65/67 Recombinant Chicken Chimeric mAb (L127/12)
Our chicken Anti-GAD65/67 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by affinity chromatography. It is great for use in ELISA, IHC, ICC, WB.



Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with GAD65/67 recombinant chicken mAb (green) (Cat No. 78-508) at 1:100 dilution. The stained sections were mounted with FluoroshieldTM mounting medium (Cat No. AR-6505). DAPI (blue) shows cell nuclei. Anti-GAD65/67 stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.
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SKU: 78-508
Ships: 1-2 business days
Product Details
GAD65/67
There are two forms of the enzyme glutamic-acid decarboxylase (GAD), a 65-kDa form (GAD65) and a 67-kDa form (GAD67) which are the result of the expression of different genes. Further splicing of GAD 67 produces one or two smaller variants, including GAD25 and GAD44. GAD67 and GAD65 are expressed in adult brain and the pancreas. The truncated forms GAD25 and GAD44 are expressed during embryonic development. Glutamic acid decarboxylase enzyme functions to catalyze the production of gamma-aminobutyric acid from L-glutamic acid.
Purified by affinity chromatography
1 mg/mL
Recombinant
L127/12
IgY
AT, ELISA, ICC, IHC, WB
Chicken
GAD1, GAD, GAD67
70 kDa
Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
ICC: 1:100
Unconjugated
Cross-reacts with GAD25, GAD44 and GAD65/GAD2
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1