Anti-VGAT Recombinant Chicken Chimeric mAb (L118/80)

Name: Anti-VGAT Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-457
Price: 470.0 USD
Availability: InStock

Our chicken Anti-VGAT recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L118/80. It detects mouse and rat VGAT, and is purified by affinity chromatography. It is great for use in ELISA, IHC, ICC, WB.

Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody (Cat No. 78-457) using goat anti-chicken IgY FITC secondary antibody (Cat No. 80-1000-FITC) (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium (Cat No. AR-6505). DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.

Western blotting of mock transfected and VGAT-FLAG transfected COS-7 lysates with anti-VGAT mouse mAb (Cat No. 75-457) and Aves Labs anti-VGAT recombinant chicken mAb (Cat No. 78-457) antibody (5 µg/mL). The mouse mAb and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. Aves Labs Recombinant Chicken anti-VGAT Mab recognizes exogenous VGAT with similar sensitivity as the anti-VGAT NeuroMab only in VGAT-FLAG transfected COS-7 lysates at ~57 kDa.

Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody (Cat No. 78-457) using goat anti-chicken IgY FITC secondary antibody (Cat No. 80-1000-FITC) (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium (Cat No. AR-6505). DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 78-457

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGAT

Target Description

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

L118/80

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc32a1 Vgat Viaat

Molecular Weight

50 kDa

Antigen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)

UniProt ID

O35633

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Anti-VGAT Recombinant Chicken Chimeric mAb (L118/80)

SKU: 78-457

Product Details

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