Anti-VGAT Recombinant Chicken Chimeric mAb (L118/80)
Our chicken Anti-VGAT recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L118/80. It detects mouse and rat VGAT, and is purified by affinity chromatography. It is great for use in ELISA, IHC, ICC, WB.



Representative FFPE section of rat brain cerebellum showing VGAT staining. The sections were stained with anti-VGAT recombinant chicken monoclonal antibody (Cat No. 78-457) using goat anti-chicken IgY FITC secondary antibody (Cat No. 80-1000-FITC) (green). The stained sections were mounted with Fluoroshield (DAPI and DABCO) mounting medium (Cat No. AR-6505). DAPI (blue) stains cell nuclei. Anti-VGAT stains the GABAergic terminals and neurons in the cerebellum. Magnification 100X.
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SKU: 78-457
Ships: 1-2 business days
Product Details
VGAT
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Purified by affinity chromatography
1 mg/mL
Recombinant
L118/80
IgY
ELISA, ICC, IHC, WB
Chicken
Slc32a1 Vgat Viaat
50 kDa
Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli
Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:500
IHC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)