Polyclonal Antibodies

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Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-AKT (Thr342) Antibody
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Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
PhosphoSolutions Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
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Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat no 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-TREM2 showing specific immunolabeling of the endogenous TREM2 at ~35kDa.
Aves Labs Anti-TREM2 Antibody
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Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.
Aves Labs Anti-Proteolipid Protein Antibody
$455
Low-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).Higher-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).
Aves Labs Anti-Integrin alpha-M (CD11b, MAC-1, OX-42 Antigen) Antibody
$455
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (1200 units/100uL lysate overnight).Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-NMDA NR2B Subunit (Ser1166) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
PhosphoSolutions Anti-GluR1-Subunit (Ser831) Antibody
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Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
PhosphoSolutions Anti-Collagen 1, alpha 1 telopeptide Antibody
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Immunolabeling of spreaded WT mouse pachytene spermatocytes positively labeling CREST/Centromere Protein (red, Cat 15-235, 1:20) and SYCP3 (green). Image kindly provided by Fernando Sánchez Sáez and Alberto M. Penás from Universidad de Salamanca, Spain.Immunostaining of Vipera berus (Reptile, snake) synaptonemal complexes identifying the lateral elements of meiotic bivalents (Anti-SYCP3, green), the centromere proteins (Anti-ACA, Cat 15-235, 1:250, red), and nuclei staining with DAPI (blue). Image kindly provided by Victor Spangenberg, Vavilov Institute of General Genetics, Moscow, Russia.
Antibodies Incorporated Anti-Centromere Protein Antibody
$170
Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.
Aves Labs Anti-Vimentin Protein Antibody
$455
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
PhosphoSolutions Anti-Phosphoserine/threonine Antibody
$329
Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53 kDa VGAT protein.
PhosphoSolutions Anti-Vesicular GABA transporter (VGAT) Antibody
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Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein.Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424916
PhosphoSolutions Anti-Troponin I (cardiac) Antibody
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Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synapsin (cat. 1926-SYNP, 1:1000, red). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I (100 µL) Antibody
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Immunostaining of HeLa cells showing specific labeling of Ki-67 (cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.
Aves Labs Anti-Ki-67 Antibody
$285
Immunohistochemical presence of tau in neurofibrillary tangles in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemical presence of tau in a neurofibrillary tangle in the remnant of a cortical neuron of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.
Aves Labs Anti-Tau Antibody
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Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. No. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (Aves Cat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.
Aves Labs Anti-Netrin-1 Antibody
$455
Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-CtIP (Thr847) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~48 kDa, ~65 kDa & ~75 kDa tau isoforms.Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-TAU antibody (cat. 1998-TAU, green, 1:2000) and anti-MAP2 (red). The blue is DAPI staining nuclear DNA. Anti-TAU labels perikarya, dendrites, axons of neurons while anti-MAP2 only labels dendrites and perikarya of neurons. Where they overlap they appear orange-yellow.
PhosphoSolutions Anti-Tau Antibody
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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
PhosphoSolutions Anti-Dopamine Transporter, C-Terminus Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.
PhosphoSolutions Anti-NMDA NR2A Subunit (100 ul) Antibody
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Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles. Photo by Dr. Gerry Shaw, Univ. Florida.GAP-43 immunoreactivity in a neuroblast within a neurosphere culture. Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500). Photomicrograph by Hoda Ilias, Aves Labs.
Aves Labs Anti-Growth-Associated Protein-43 Antibody
$285
A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.
Aves Labs Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody
$455

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