Anti-AKT (Thr342) Antibody
Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Product Specific References for Applications and Species
- Western Blot: Drosophila | Mosquito
| Western Blot: Drosophila | ||
| PMID | Dilution | Publication |
| 33370287 | 1:500 | Towler, B.P., et al. 2020. Dis3L2 regulates cell proliferation and tissue growth though a conserved mechanism. PLoS Genetics, 16(12), p.e1009297. |
| 32291952 | 1:1000 | Woodling, N.S., et al. 2020. The neuronal receptor tyrosine kinase Alk is a target for longevity. Aging Cell, p.e13137. |
| 31061470 | 1:500 | Xu, Y., et al. 2019. Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific Reports, 9(1), p.6989. |
| 29892262 | 1:1000 | Post, S., et al. 2018. Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28; 9:245. |
| 29491510 | not listed | Lin, F., et al. 2017. Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212. |
| 25043021 | 1:200 | Schleich, S., et al. 2014. DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth. Nature. Aug 14; 512(7513):208-12. |
| Western Blot: Mosquito | ||
| PMID | Dilution | Publication |
| 37913852 | 1:1000 | Chen, K, et al. 2023. Insulin-like peptides and ovary ecdysteroidogenic hormone differentially stimulate physiological processes regulating egg formation in the mosquito Aedes aegypti. Insect biochemistry and molecular biology, 104028. |
| 35129195 | 1:1000 | Martinson, E., et al. 2022. Insulin-like peptide 3 stimulates hemocytes to proliferate in anautogenous and facultatively autogenous mosquitoes. The Journal of Experimental Biology, 225(5). |
Product Specific Protocols
- Western Blot Protocol: Download