Polyclonal Antibodies

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Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.
Mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining). Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.
Beta-Amyloid-positive “neuritic plaque” (shown in higher magnification) in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer’s disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemistry of a capillary showing beta-amyloid peptide in the wall of the vessel. Paraffin-embedded section of a human brain from a patient with diagnosed Alzheimer's Disease. Primary antibody -- 1:500; secondary antibody -- 1:1000 HRP-Goat anti-chicken IgY (Aves Labs). Dr. Randy Woltjer, Dept Pathology, OHSU
Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.

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