Polyclonal Antibodies

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Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Dopamine Transporter (Thr53) Antibody
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Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
PhosphoSolutions Anti-Microtubule Associated Protein 2 (MAP2) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Effects of striatal slice treatment with NMDA (25 μM, 5 min), dopamine (DA, 10 μM, 15 min), or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4.0. PMID: 35835216
PhosphoSolutions Anti-GluR1-Subunit (Ser845) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1472) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT. Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Aves Labs anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.
Aves Labs Anti-SOX2 Antibody
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Doublecortin (1:1000 dilution, green) staining of neuroblastoma cells in culture. Red staining is a Golgi apparatus marker. Page Balisch (University of Arizona).Doublecortin (1:1000 dilution, green) staining of a tissue section (4% paraformaldehyde-fixed, paraffin- embedded) through the cochlear ganglion from a neonatal mouse. Red staining is neurofilament, NF-M, visualized with Texas Red-goat anti-rabbit IgG.
Aves Labs Anti-Doublecortin Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71 kDa FMRP protein phosphorylated at Ser499 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-FMRP (Ser499) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0.
PhosphoSolutions Anti-CaM Kinase II (Thr286) Antibody
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Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Aves Labs Anti-Glutamic Acid Decarboxylase-67 Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr306 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 minutes).
PhosphoSolutions Anti-CaM Kinase II (Thr306) Antibody
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Western blot of HEK293 lysate showing specific immunolabeling of the ~160 kDa MerTK phosphorylated at Tyr749/753/754 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 60 minutes).Western blot image of human THP-1 cells treated with γ-Carboxylated Gas6 at a 10 nM concentration showing increased MerTK phosphorylation (Cat no p186-749). Image from publication CC-BY-4.0. PMID: 38673989
PhosphoSolutions Anti-MerTK (Tyr749/753/754) Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
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Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.
PhosphoSolutions Anti-Tyrosine Hydroxylase (Ser40) Antibody
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Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).
Aves Labs Anti-Peripherin Antibody
$285
Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.
Aves Labs Anti-DYKDDDDK Epitope Tag/FLAG Antibody
$295
Immunofluorescent staining of COS-7 cells expressing S100B-flag using Aves Labs anti-S100B antibody (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex). Sections were stained with Aves Labs anti-S100B antibody at 1:500 dilution and detected with anti-chicken HRP.
Aves Labs Anti-S100B Antibody
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Immunocytochemical staining of mouse brain cortical neurons in culture. NFL immunoreactivity is red and yellow. Blue staining is DAPI nuclear staining. Anti-NFL (1:1000 dilution). Dr. Felix Eckenstein, University of Vermont.Immunohistochemical staining of a paraffin-embedded section through mouse cerebellum. Anti-NFL immunoreactivity (Aves Labs, 1:500 dilution) was detected using HRP-labeled goat anti-chicken IgY (Aves Cat.No. H-1004).
Aves Labs Anti-NFL (Neurofilament Light Chain) Antibody
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Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (cat.1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP ( cat.1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
PhosphoSolutions Anti-Microtubule Associated Protein 2 (MAP2) Antibody
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Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Aves Labs Anti-V5 Antibody
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Representative images of heterozygous (top) and homozygous (bottom) fibroblasts stained with AURKB (C, prophase) or survivin (metaphase, D) (red; shown in grayscale) and FITC-conjugated CREST (cat. 15-235-F, 1:100, green; shown in grayscale). Image from publication CC-BY-4.0. PMID:28272472Immunofluorescence staining of fibroblasts shows partial and transient localization of WDR62 (red) in prometaphase kinetochores (CREST, cat. 15-235-F, 1:100). Image from publication CC-BY-4.0. PMID:28272472
Antibodies Incorporated Anti-Centromere Protein Antibody - FITC Labeled
$260
Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) and Vimentin (blue) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.
Aves Labs Anti-Synaptotagmin-1 Antibody
$455
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-GABAA Receptor, ß3 (Ser408/409) Antibody
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Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
PhosphoSolutions Anti-Aquaporin 2 (Ser269) Antibody
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