Anti-GluR1-Subunit (Ser831) Antibody

22 Citations

Our Anti-GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
Representative Immunoblots and quantification of in vitro kinase reactions at 30 °C with increasing ATP concentration measuring purified CaMKIIα phosphorylation of GST-GluA1 S831 (cat. p1160-831, 1:2000). Image from publication CC-BY-4.0. PMID: 37648853
Immunoblots of in vitro kinase reactions at 30 °C including GFP–CaMKII WT, K42M and F89G with GST–GluA1-c tail showing specific labelling of GluR1 S831 (cat. p1160-831, 1:2000). Reactions were performed either with vehicle control or with 10 μM NM-PP1. Image from publication CC-BY-4.0. PMID: 37648853
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
Representative Immunoblots and quantification of in vitro kinase reactions at 30 °C with increasing ATP concentration measuring purified CaMKIIα phosphorylation of GST-GluA1 S831 (cat. p1160-831, 1:2000). Image from publication CC-BY-4.0. PMID: 37648853
Immunoblots of in vitro kinase reactions at 30 °C including GFP–CaMKII WT, K42M and F89G with GST–GluA1-c tail showing specific labelling of GluR1 S831 (cat. p1160-831, 1:2000). Reactions were performed either with vehicle control or with 10 μM NM-PP1. Image from publication CC-BY-4.0. PMID: 37648853

Mouse, Rat
WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p1160-831

Volume: 150 µL
Price:
$430.00

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
22427672 not listed Jakan-Sakrikar, N., et al. 2012. Substrate-selective and calcium-independent activation of CaMKII by α-actinin. Journal of Biological Chemistry, 287(19), pp.15275-15283.
21610080 not listed Jiao, Y., et al. 2011. Characterization of a Central Ca2+/Calmodulin-dependent Protein Kinase IIα/β Binding Domain in Densin That Selectively Modulates Glutamate Receptor Subunit Phosphorylation. Journal of Biological Chemistry 286: 24806 - 24818.
Western Blot: Mouse
PMID Dilution Publication
376488531:2000Tullis, JE, et al. 2023. LTP induction by structural rather than enzymatic functions of CaMKII. Nature, 146-153.
33853773 1:1000 Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300.
26255006 1:500 Pasek, J.G., et al. 2015. Differential CaMKII regulation by voltage-gated calcium channels in the striatum. Molecular and Cellular Neuroscience, 68, 234-243.
24907515 not listed Mao, L.M., et al. 2014. Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European Journal of Pharmacology, 738, 237-244.
20554878 1:500 Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. Journal Neuroscience, 30: 8263-8273.
17923095 not listed Hu,H., et al. 2007. Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173.
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Western Blot: Rat
PMID Dilution Publication
31994358 1:500 Mao, L.M., et al. 2018. Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain. Brain and Behavior, p.e01543.
29432883 not listed Mao, L.M., et al. 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93.
29242119 not listed Xue, B., et al. 2017. Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European Journal of Pharmacology. 2017 Dec 11;820:45-52.
28578480 not listed Lameth, J., et al. 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16.
27060412 not listed Xue, B., et al. 2017. Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65.
26706598 not listed O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of Disease, 87, 134-144.
26658861 1:1000 Bean, L.A., et al. 2015. Re-opening the critical window for estrogen therapy. Journal of Neuroscience, 35(49), pp.16077-16093.
25689263 1:1000 Mao, L.M., et al. 2015. Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of Neurochemistry. Jun;133(6):795-805.
24780380 1:500 Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12.
24231469 1:1000 Xue, B., et al. 2014. Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry International, 64, 48-54.
24056996 1:1000 Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Molecular Pharmacology, 84(6), 834-843.
20554878 1:500 Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263 - 8273.
20353941 not listed Coultrap, S.J., et al. 2010. CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. Journal of Biological Chemistry, 285: 17930 – 1793.
18819923 1:1000 Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. Journal of Biological Chemistry, 283: 33138 - 33146.
18519737 not listed Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831 - 846.

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