Anti-V5 Antibody

Name: Anti-V5 Antibody
Brand: Aves Labs
SKU: ET-V5-0100
Price: 455.0 USD
Availability: InStock

Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects V5, and is affinity purified. It is great for use in ICC, WB.

Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.

Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.

Western blot of HEK293 cells transfected with V5-tagged CASR (lane 1) or mock transfected cells (lane 2) using chicken α-V5 antibody showing specific immunolabeling at the expected molecular weight

Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: ET-V5-0100

Volume: 500 µL

Variant

Price:

$455.00

Quantity:

Product Details

Target

Target Description

The V5 epitope tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

Format

Affinity purified

Concentration

0.2mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Antigen

Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

Antibody Registry ID

AB_2910559

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

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Anti-V5 Antibody

SKU: ET-V5-0100

Product Details

Bulk Order Anti-V5 Antibody

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