Anti-NFL (Neurofilament Light Chain) Antibody
Three antipeptide antibodies were generated in chickens against sequences shared between the mouse (P08551) and human (P07196) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
SKU: NFL
Product Details
Neurofilament Light Chain (NFL)
Human Neurofilament Light Chain (NFL) is a 61,516 dalton protein (543 amino acids) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NFL gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F).
Affinity-Purified IgY
300 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
NEFL NF68 NFL
70 kDa
Synthetic peptides
Human, Mouse, Rat
AB_2313553
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NF-L gene product, but are shared between the human (P07196, NCBI) and mouse (P08551, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Neurofilament light polypeptide (NF-L) (68 kDa neurofilament protein) (Neurofilament triplet L protein)
UniProt (Immunogen Species): P07196
Product Specific References for Applications and Species
Immunocytochemistry: Mouse | ||
PMID | Dilution | Publication |
29404402 | 1:500 | Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, . |
Immunocytochemistry: Rat | ||
PMID | Dilution | Publication |
30038033 | 1:500 | Lee, S.J., et al. 2018. hnRNPs interacting with mRNA localization motifs define axonal RNA regulons. Molecular & Cellular Proteomics, 2091. |
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
28828403 | 1:1,000 | Lerch, J.K., et al. 2018. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs. eNeuro, . |