Anti-Glutamic Acid Decarboxylase-67 Antibody

2 Citations

This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.

Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).
Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500). Hoda Ilias, Aves Labs.
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500). Hoda Ilias, Aves Labs.
Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).
Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).
Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500). Hoda Ilias, Aves Labs.
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500). Hoda Ilias, Aves Labs.

Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet

SKU: GAD

Volume: 1000 µL
Price:
$455.00

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
283775851:2000Bigler, R.L., et al. 2017. Messenger RNAs localized to distal projections of human stem cell derived neurons. Nature Scientific Reports, 611.
Immunocytochemistry: Rat
PMID Dilution Publication
289318111:2000Nagendran, T., et al. 2017. Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling. Natrue Communications, 625.

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