Anti-NMDA NR2B Subunit (Ser1166) Antibody

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Our Anti-NMDA NR2B Subunit (Ser1166) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Ser1166) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (1200 units/100uL lysate overnight).
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (1200 units/100uL lysate overnight).
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (1200 units/100uL lysate overnight).
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Mouse, Rat
WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p1516-1166

Volume: 100 µL
Price:
$430.00

Product Specific References for Applications and Species

Western Blot: Mouse
PMID Dilution Publication
25158771 not listed Knox, R., et al. 2014. NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), pp.3040-3047.
21464302 1:1000 HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.
Western Blot: Rat
PMID Dilution Publication
33744339 1:1000 Chen, W., et al. 2021. cAMP signaling through protein kinase A and Epac2 induces substance P release in the rat spinal cord. Neuropharmacology, p.108533.
21464302 1:1000 HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.

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