Anti-Nestin Antibody

Name: Anti-Nestin Antibody
Brand: Aves Labs
SKU: NES
Price: 455.0 USD
Availability: InStock

Three antipeptide antibodies were generated in chickens against a sequence in mouse (NP_057910) gene product. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Immunocytochemistry showing nestin immunoreactivity (green; 1:1000 dilution) in A7r5 neuroblastoma cells in culture. Blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of A7r5 neuroblastoma cells in culture. Nestin immunoreactivity (red staining, Aves Labs, 1:1000 dilution; green is β-Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). Green is nestin immunoreactivity (1:1000 dilution). Blue is DAPI nuclear staining. Hoda Ilias, Aves Labs.

Nestin Western Blot. 20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa. Hoda Ilias, Aves Labs.

Coronal sections of E12.5 p130Cas::BacEGFP cortices stained for the RGC marker Nestin (cat. NES, 1:500; magenta) and EGFP (green). Counterstain is DAPI (blue). Image from publication CC-BY-4.0. PMID:37540708

Coronal sections of E15.5 p130Cas::BacEGFP cortices stained for the RGC marker Nestin (cat. NES, 1:500; magenta) and EGFP (green). Counterstain is DAPI (blue). Image from publication CC-BY-4.0. PMID:37540708

Laminin (green) and Nestin (NES, 1:500; magenta) antibody staining on E15.5 control and Emx1Cre;CasTcKO coronal sections. Radial glial endfeet do not make proper contact with the basal lamina in Emx1Cre;CasTcKO animals at exposed Laminin sites (arrows). Image from publication CC-BY-4.0. PMID:37540708

Immunofluorescence for pY165Cas (cyan), c-Myc (magenta), and Nestin (cat. NES, 1:500; blue) of WT and Emx1Cre;β1-Integrinflox/flox mixed cortical cultures transfected with a control vector (myc) or a construct overexpressing myc-tagged full-length Dag1 (myc-Dag1). Counterstain is DAPI (yellow). Image from publication CC-BY-4.0. PMID:37540708

Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: NES

Volume: 1000 µL

Variant

Price:

$455.00

Quantity:

Product Details

Target

Nestin

Target Description

Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Format

Affinity-Purified IgY

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NES Nbla00170

Molecular Weight

250 kDa

Antigen

Synthetic peptides

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2314882

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Nestin

UniProt Details

UniProt (Immunogen Species): P48681

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse| Rat
Immunohistochemistry: Human | Mouse | Rat
Western Blot: Mouse
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
31075188	1:200	Luzuriagaa, J., et al. 2019. BDNF and NT3 Reprogram Human Ectomesenchymal Dental Pulp Stem Cells to Neurogenic and Gliogenic Neural Crest Progenitors Cultured in Serum-Free Medium. Cellular Physiology and Biochemistry, 1361-1380.
30713029	1:500	Hongda, L., et al. 2019. Zika Virus Protease Cleavage of Host Protein Septin- 2 Mediates Mitotic Defects in Neural Progenitors. Neuron, 1089-1098.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37540708	1:500	Wong, W, et al. 2023. An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold. PLoS Biology, e3002212.
36764294	not listed	Zhang, F., et al. 2023. Epitranscriptomic Regulation of Cortical Neurogenesis via Mettl8-Dependent Mitochondrial tRNA m3C Modification. Cell Stem Cell, 300-311.
35735108	not listed	Dai, S., et al. 2022. Dynamic profiling and functional interpretation of histone Kcr and Kla during neural development. Development, dev200049.
34561615	1:400	Kerloch, T., et al. 2021. The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis. Molecular Psychiatry, 7280-7295.
33622776	1:600	Little, J.N., et al. 2021. Loss of Coiled-Coil Protein Cep55 Impairs Neural Stem Cell Abscission and Results in p53-Dependent Apoptosis in Developing Cortex. Journal of Neuroscience, 3344-3365.
32273386	1:1000	McNeely, K.C., et al. 2020. Cytokinesis and postabscission midbody remnants are regulated during mammalian brain development. PNAS, 9584-9593.
30840538	1:300	Bott, C.J., et al. 2019. Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.. Molecular Biology of the Cell, 1214-1229.
28154169	1:500	Su, W., et al. 2017. CD44 and Hyaluronan Regulate Adult Hippocampal Neural Stem Cell Quiescence and Differentiation. Journal Biochemical Chemistry, 4434-4445.
26439128	1:1000	Malik, A., et al. 2016. Circadian Clock Genes Are Essential for Normal Adult Neurogenesis, Differentiation, and Fate Determination. Plos One, e0139655.
25826427	1:1000	Malik, A., et al. 2015. Development of circadian oscillators in neurosphere cultures during adult neurogenesis. PLoS One, e0122937.
25674053	1:2000	Zheng, L.S., et al. 2015. Minocycline treatment ameliorates interferon-alpha-induced neurogenic defects and depression-like behaviors in mice. Frontiers in Cellular Neuroscience, 5.
20735994	1:2000	Saito, K., et al. 2010. Moderate low temperature preserves the stemness of neural stem cells and suppresses apoptosis of the cells via activation of the cold-inducible RNA binding protein. Brain Research, 20-29.
20151365	1:100	White, R.E., et al. 2010. Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice.. J Comp Neurol, 1370-1390.

Immunocytochemistry: Rat
PMID	Dilution	Publication
20403075	1:5000	Hahn, Y.K., et al. 2010. β‐Chemokine production by neural and glial progenitor cells is enhanced by HIV‐1 Tat: effects on microglial migration. Journal of Neurochemistry, 97-109.

Immunohistochemistry: Human
PMID	Dilution	Publication
28556287	1:500	Ho, C.Y., et al. 2017. Differential Neuronal Susceptibility and Apoptosis in Congenital ZIKV Infection. Annals of Neurology, 121-127.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38977310	1:1000	Pastor-Alonso, O., et al. 2024. HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures. Life Science Alliance, .
38971831	1:500	Zhao, T., et al. 2024. Epigenetic maintenance of adult neural stem cell quiescence in the mouse hippocampus via Setd1a. Nature Communications, 5674.
37540708	1:500	Wong, W, et al. 2023. An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold. PLoS Biology, e3002212.
37156778	1:200	Li, Y., et al. 2023. Neutralization of excessive levels of active TGF-β1 reduces MSC recruitment and differentiation to mitigate peritendinous adhesion. Bone Research, 24.
37054704	1:500	Sun, X.L., et al. 2023. Stem Cell Competition Driven by the Axin2-p53 Axis Controls Brain Size During Murine Development. Developmental Cell, 744-759.
36089914	1:200	Luo, W., et al. 2022. Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation. Glia, 284-304.
35963237	1:500	Chi, S., et al. 2022. Astrocytic Piezo1-mediated mechanotransduction determines adult neurogenesis and cognitive functions. Neuron, 2984-2999.
35328825	not listed	Rodríguez-Iglesias, N., et al. 2022. Susceptibility of Female Mice to the Dietary Omega-3/Omega-6 Fatty-Acid Ratio: Effects on Adult Hippocampal Neurogenesis and Glia. International journal of molecular sciences, 3399.
34982030	1:400	Guo, N., et al. 2022. Transcriptional regulation of neural stem cell expansion in the adult hippocampus. Elife, e72195.
34562292	not listed	Liu, X., et al. 2021. Arid1a regulates neural stem/progenitor cell proliferation and differentiation during cortical development. Cell Proliferation, e13124.
34526402	1:500	Doi, H., et al. 2021. Early-life midazolam exposure persistently changes chromatin accessibility to impair adult hippocampal neurogenesis and cognition. PNAS: USA, e2107596118.
33646122	1:300	Sun, Q., et al. 2021. Parathyroid hormone attenuates osteoarthritis pain by remodeling subchondral bone in mice. Elife, e66532.
33338666	not listed	Khalid, A.B., et al. 2021. GATA4 regulates mesenchymal stem cells via direct transcriptional regulation of the WNT signalosome. Bone, 115819.
32068188	1:200	Matuzelski, E., et al. 2020. Expression of NFIA and NFIB within the murine spinal cord. Gene Expression Patterns, 119098.
31059735	1:1000	Beligala, D.H., et al. 2019. Musashi-2 and related stem cell proteins in the mouse suprachiasmatic nucleus and their potential role in circadian rhythms. International Journal of Developmental Neuroscience, 44-58.
30929900	not listed	Berg, D.A., et al. 2019. A Common Embryonic Origin of Stem Cells Drives Developmental and Adult Neurogenesis.. Cell Reports, 654-668.
30840538	1:400	Bott, C.J., et al. 2019. Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.. Molecular Biology of the Cell, 1214-1229.
30742117	1:500	Shen, M., et al. 2019. Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice. Nature Neuroscience, 386-400.
30304535	1:600	Neville Little, J., et al. 2019. p53 deletion rescues lethal microcephaly in a mouse model with neural stem cell abscission defects. Human Molecular Genetics, 434-447.
30355452	1:250	Cheng, C.C., et al. 2018. Hair follicle epidermal stem cells define a niche for tactile sensation. eLIFE, e38883.
30174295	1:250	Zhou, Y., et al. 2018. Autocrine Mfge8 Signaling Prevents Developmental Exhaustion of the Adult Neural Stem Cell Pool.. Cell Stem Cell, 444-452.
30004576	not listed	Yamada, S., et al. 2018. Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system. Journal of Comparative Neurology, 2099-2114.
29684005	1:500	Wong, S.Z.H., et al. 2018. In vivo clonal analysis reveals spatiotemporal regulation of thalamic nucleogenesis. Plos One, e2005211.
29478897	1:1000	Crowther, A.J., et al. 2018. An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. Stem Cell Reports, 1146-1159.
29463313	not listed	Xiao, X., et al. 2018. Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells. Journal of Neuroinflammation, 49.
29416028	1:300	Wang, X., et al. 2018. Inhibition of overactive TGF-β attenuates progression of heterotopic ossification in mice. Nature Communications, 551.
29355842	1:300	Wang, X., et al. 2018. Aberrant TGF-β activation in bone tendon insertion induces enthesopathy-like disease. Journal of Clinical Investigation, 846-860.
29292230	1:10,000	Huang, H., et al. 2018. Bone resorption deficiency affects tooth root development in RANKL mutant mice due to attenuated IGF-1 signaling in radicular odontoblasts. Bone, 161-171.
29276142	1:1000	Jinnou, H., et al. 2018. Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell, 128-137.
29101351	1:100	Li, C., et al. 2017. Programmed cell senescence in skeleton during late puberty. Nature Communications, 1312.
29089377	1:1000	Nakamuta, S., et al. 2017. Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain. Journal of Cell Biology, 4313-4330.
28826723	not listed	Yoon, K.J., et al. 2017. Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins. Cell Stem Cell, 349-358.
28771884	1:500	Kimura, A., et al. 2017. HMGB2 expression is associated with transition from a quiescent to an activated state of adult neural stem cells. Developmental Dynamics, 229-238.
28642362	not listed	Renault-Mihara, F., et al. 2017. Regulation of RhoA by STAT3 coordinates glial scar formation. Journal of Cell Biology, 2533-2550.
28154169	1:500	Su, W., et al. 2017. CD44 and Hyaluronan Regulate Adult Hippocampal Neural Stem Cell Quiescence and Differentiation. Journal Biochemical Chemistry, 4434-4445.
28100736	1:500	Jobe, E.M., et al. 2016. Methyl-CpG binding protein MBD1 regulates neuronal lineage commitment through maintaining adult neural stem cell identity. The Journal of Neuroscience, 523-536.
27778125	not listed	Shimozaki, K., et al. 2016. Ten-Eleven Translocation 1 and 2 Confer Overlapping Transcriptional Programs for the Proliferation of Cultured Adult Neural Stem Cells. Cellular and Molecular Neurobiology, 995-1008.
27256073	1:300	Bian, Q., et al. 2016. Excessive Activation of TGFβ by Spinal Instability Causes Vertebral Endplate Sclerosis. Nature Scientific Reports, 27093.
26837060	1:300	Xie, L., et al. 2016. Systemic neutralization of TGF‐β attenuates osteoarthritis. Annals of the New York Academy of Sciences, 53-64.
26209457	1:2000	Langness, S., et al. 2015. Vagus Nerve Mediates the Neural Stem Cell Response to Intestinal Injury. Journal of the American College of Surgeons, 871-879.
25068123	1:500	Zheng, L.S., et al. 2014. Mechanisms for interferon-α-induced depression and neural stem cell dysfunction. Stem Cell Reports, 73-84.
24639124	not listed	Hasegawa, Y., et al. 2014. Spatiotemporal Distribution of SUMOylation Components during Mouse Brain Development. The Journal of Comparative Neurology Research in Systems Neuroscience, 3020-3036.
23831515	1:500	Yuniarti, N., et al. 2013. Prenatal exposure to suberoylanilide hydroxamic acid perturbs corticogenesis. Neuroscience Research, 42-49.
22865725	1:5000	Hahn, Y.K., et al. 2012. HIV‐1 alters neural and glial progenitor cell dynamics in the central nervous system: Coordinated response to opiates during maturation. Glia, 1871-1887.
21245095	1:250	Pierfelice, T.J., et al. 2011. Notch3 activation promotes invasive glioma formation in a tissue site-specific manner. Cacncer Research, 1115-1125.
20151365	1:500	White, R.E., et al. 2010. Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice.. J Comp Neurol, 1370-1390.

Immunohistochemistry: Rat
PMID	Dilution	Publication
21655322	1:1000	Guo, W., et al. 2011. Alcohol exposure decreases CREB binding protein expression and histone acetylation in the developing cerebellum. Plos One, e19351.

Western Blot: Mouse
PMID	Dilution	Publication
30840538	1:3000	Bott, C.J., et al. 2019. Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.. Molecular Biology of the Cell, 1214-1229.
30004576	1:4,000	Yamada, S., et al. 2018. Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system. Journal of Comparative Neurology, 2099-2114.

Additional Publications: Unspecified
PMID	Publication
35811097	Pineda, J., et al. 2022. In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering. Methods in Cell Biology, 147-167.
31340161	Ogawa, Y., et al. 2019. Abnormal organization during neurodevelopment in a mouse model of Sandhoff disease.. Neurosci Res, 12-19.
26946195	Reeve, R.L., et al. 2016. Targeted activation of primitive neural stem cells in the mouse brain. European Journal of Neuroscience, 1474-1485.
26012865	Wei, W., et al. 2015. CD38 is required for neural differentiation of mouse embryonic stem cells by modulating reactive oxygen species. Stem Cells, 2664-2673.
24123239	Fiorelli, R., et al. 2013. The adult spinal cord harbors a population of GFAP‐positive progenitors with limited self‐renewal potential. Glia, 2100-2113.
23542688	Bardehle, S., et al. 2013. Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation. Nature Neuroscience, 580-586.

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Anti-Nestin Antibody

SKU: NES

Product Details

Product Specific References for Applications and Species

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