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Immunolabeling of postnatal 14 mouse CA3 specifically labeling PSD93 (Cat no 75-057, 1:400, green) and Ankyrin G(red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50uM slices were floating sections. Objective 25x, scale bar = 20um. Images kindly provided by Jorge Ramirez-Franco and Oussama El Far, Aix-Marseille Université and INSERM. Immunoblot against crude membranes from whole adult rat brain (RBM) or hippocampi (MHM) from adult PSD-93/Chapsyn-110 knockout (KO) or wild-type (WT) mice. Mouse samples courtesy of Richard Huganir (Johns Hopkins University).
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.
Adult rat brain immunohistochemistry.

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