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Immunoblot against isolated cilia and extracts of COS cells transiently transfected with GFP-tagged DNAIC1 or untagged Kv2.1 plasmid probed with UNC 65.56.18.11 (left) or N52A/42 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L, DNMT3A and DNMT3B plasmids and probed with N117/9 TC supe (left) and mouse anti-Flag (right).Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids and probed with N150/21 (left), N86/8 (middle) and K89/41 (right).
Adult rat striatum immunohistochemistry.
Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.

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