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COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.Western blotting of mock or NDRG2-FLAG transfected COS-7 cell lysates and human, mouse, and rat brain homogenates (10 ug/lane) with Aves Labs chicken anti-NDRG2 antibody (0.2 ug/ml) and detected with anti-chicken HRP. Anti-NDRG2 recognizes endogenous NDRG2 in brain homogenates in addition to exogenous NDRG2 in COS-7 cells.
Aves Labs Anti-NDRG2 Antibody
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Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.
Aves Labs Anti-Nestin Antibody
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Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. No. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (Aves Cat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.
Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, green; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, red, 1:1000 dilution).Immunohistochemical photomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, green; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, red, 1:1000 dilution).
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Neurexin-1-Alpha, Neurexin-1- Beta or untagged Kv2.1 plasmid and probed with N170A/1 TC supe (left) and rabbit anti-Flag (right).
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.

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