Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY Secondary Antibody
Our Alkaline Phosphatase-Labeled Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.
SKU: AP-1001
Product Details
Chicken IgY
Affinity Purified
0.1 mg/mL
Polyclonal
IgG
Goat
IgY
Chicken
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Liquid
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to alkaline phosphatase.
HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), MgCl2 (1.0 mM), ZnCl2 (0.1 mM), sodium azide (0.02%, w/v) (as an antimicrobial agent).
AP
Does not cross-react with mammalian IgGs
Antibodies were analyzed in a sandwich ELISA format where a 1:800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-nitrophenyl phosphate as the substrate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
Ice packs
Product Specific References for Applications
Western Blot | ||
PMID | Dilution | Publication |
18658160 | not listed | Tollner, T.L., et al. 2008. Macaque sperm coating protein DEFB126 facilitates sperm penetration of cervical mucus. Human Reproduction, 2523-2534. |