Anti-Epithelial Cell Adhesion Molecule (Ep-CAM) Antibody (ESA 214)

Name: Anti-Epithelial Cell Adhesion Molecule (Ep-CAM) Antibody
Brand: Antibodies Incorporated
SKU: MM-1014
Price: 319.0 USD
Availability: InStock

Our Anti-Epithelial Cell Adhesion Molecule mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone ESA 214. It detects human Epithelial Cell Adhesion Molecule, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB, ELISA.

Western blotting of SW1116 cell lysate using 0.2 ug/mL, 1 ug/mL, or 5 ug/mL mouse monoclonal anti-Epithelial Specific Antigen (Ep-CAM) antibody (MM-1014). MM-1014 mouse anti-Ep-CAM antibody recognizes endogenous Ep-CAM at 40 kDa.

Immunofluorescent staining of MCF7 cells using 10 ug/mL mouse monoclonal anti-EpCAM antibody (MM-1014) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat#AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-EpCAM stains the membrane of MCF7 cells.

Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: MM-1014

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Epithelial Cell Adhesion Molecule

Target Description

Epithelial specific antigen is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM). This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the “Cancer Stem Cells” (CSC) theory, tumors are not viewed as simple monoclonal expression of transformed cells, but rather as complex tissue where abnormal growth is driven by a minority pathological CSC pool that on one hand has acquired tumor related features such as uncontrolled growth and ability to form metasis and on the other hand maintains its inherent capacity to self renew and differentiate into a phenotypic ally heterogeneous, although aberrant progency. This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells.

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

ESA 214

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

EPCAM

Molecular Weight

40 kDa

Antigen

Small cell lung carcinoma

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ep-CAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1

UniProt Details

UniProt (Human): P16422
UniProt (Immunogen Species): P16422

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Anti-Epithelial Cell Adhesion Molecule (Ep-CAM) Antibody (ESA 214)

SKU: MM-1014

Product Details

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