Anti-CNGA1 and CNGA3 Antibody (L36/12)

Name: Anti-CNGA1 and CNGA3 Antibody
Brand: NeuroMab
SKU: 75-107
Price: 319.0 USD
Availability: InStock

12 Citations

Our Anti-CNGA1 and CNGA3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L36/12. It detects mouse and rat CNGA1 and CNGA3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Crude lysate of 7-day old zebrafish larvae immunoblot.

Isolated goldfish cone immunohistochemistry. Data courtesy of Diane Henry-Vanisko and Gary Matthews.

Zebrafish retina immunohistochemistry. Data courtesy of Diane Henry-Vanisko and Gary Matthews.

Rat retina immunohistochemistry. Data courtesy of Diane Henry-Vanisko and Gary Matthews.

Crude lysate of 7-day old zebrafish larvae immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-107

Product Details

Target

CNGA1 and CNGA3

Target Description

This antibody recognizes both Cyclic Nucleotide Gated Channel Subunit Alpha 1 and Cyclic Nucleotide Gated Channel Subunit Alpha 3, which are both members of the cyclic nucleotide-gated cation channel family. These ion channels function in response to the binding of cyclic nucleotides. CNG channels are nonselective cation channels that are found in the membranes of various tissue and cell types, and are significant in sensory transduction as well as cellular development.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L36/12

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Cnga3 Cng3

Molecular Weight

80 kDa (size will vary depending on species and isoform)

Antigen

Fusion protein amino acids 535-637 (cytoplasmic C-terminus) of goldfish CNG (accession number AAO16601) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10672375

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.35

Dilution Ranges

IHC: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Cyclic nucleotide-gated cation channel alpha-3 (Cone photoreceptor cGMP-gated channel subunit alpha) (Cyclic nucleotide-gated channel alpha-3) (CNG channel alpha-3) (CNG-3) (CNG3)

UniProt Details

UniProt (Human): Q9HCJ2
UniProt (Immunogen Species): AAO16601

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
19778592	not listed	Khan S, et al. 2010. A novel cyclic nucleotide-gated ion channel enriched in synaptic terminals of isotocin neurons in zebrafish brain and pituitary.. Neuroscience, 79-89.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33711342	1:1000	Sharif AS, et al. 2021. Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis. Journal of Biological Chemistry, 100529.
30573647	1:1000	Dilan TL, et al. 2019. ARL13B, a Joubert Syndrome-Associated Protein, Is Critical for Retinogenesis and Elaboration of Mouse Photoreceptor Outer Segments. Journal of Neuroscience, 1347-1364.
30446707	1:1000-1:2000	Wright ZC, et al. 2018. ADP-Ribosylation Factor-Like 2 (ARL2) regulates cilia stability and development of outer segments in rod photoreceptor neurons. Scientific Reports, 16967.
30228185	1:1000	Ying, et al. 2018. The small GTPase RAB28 is required for phagocytosis of cone outer segments by the murine retinal pigmented epithelium. Journal of Biological Chemistry, 17546-17558.
27613864	1:1000	Murphy J, et al. 2016. Palmitoylation of Progressive Rod-Cone Degeneration (PRCD) Regulates Protein Stability and Localization. Journal of Biological Chemistry, 23036-23046.
27529348	not listed	Ying G, et al. 2016. Small GTPases Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors. PLoS One, e0161236.
27147662	not listed	Christiansen JR, et al. 2016. Deficiency of Isoprenylcysteine Carboxyl Methyltransferase (ICMT) Leads to Progressive Loss of Photoreceptor Function. Journal of Neuroscience, 5107-5114.
26814127	1:500	Hanke-Gogokhia C, et al. 2016. Arf-like Protein 3 (ARL3) Regulates Protein Trafficking and Ciliogenesis in Mouse Photoreceptors.. The Journal of Biological Chemistry, 7142-7155.
24790208	1:2000	Ying G, et al. 2014. Centrin 2 is required for mouse olfactory ciliary trafficking and development of ependymal cilia planar polarity.. Journal of Neuroscience, 6377-6388.
21555557	not listed	Christiansen JR, et al. 2011. RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking of rod phosphodiesterase 6 to photoreceptor outer segments.. PNAS: USA, 8862-8866.

Western Blot: Mouse
PMID	Dilution	Publication
26936825	not listed	Wright ZC, et al. 2016. ARL3 regulates trafficking of prenylated phototransduction proteins to the rod outer segment. Human Molecular Genetics, 2031-2044.

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Anti-CNGA1 and CNGA3 Antibody (L36/12)

SKU: 75-107

Product Details

Product Specific References for Applications and Species

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