Anti-GABA-A-R-Alpha4 Antibody (N398A/34)
Our Anti-GABA-A-R-Alpha4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N398A/34. It detects mouse and rat GABA-A-R-Alpha4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
SKU: 75-383
Product Details
GABA-A-R-Alpha4
Gamma-aminobutyric acid receptor subunit alpha-4 or GABA AR alpha4 is encoded by the gene GABRA4. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 4 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha 4 has been found to be expressed in skeletal muscle tissue, adult mammalian kidney, heart and primarily in brain at the cell junction, synapse,and the postsynaptic cell membrane. Disorders involving the GABRA4 gene include autism, Status Epilepticus and restless legs syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N398A/34
IgG1
ICC, IHC, WB
Mouse
Gabra4
60 kDa
Fusion protein amino acids 410-450 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha4 (accession number P28471) produced recombinantly in E. Coli
Mouse, Rat
AB_2341108
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:500
IHC: 1:250
IHC: 1:250
Unconjugated
Does not cross-react with other GABA-A receptor Alpha subunits
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4)
UniProt (Human): P48169
UniProt (Immunogen Species): P28471
UniProt (Immunogen Species): P28471
Product Specific References for Applications and Species
Immunocytochemistry: Rat | ||
PMID | Dilution | Publication |
34546336 | 1:250 | Kreye, J., et al. 2021. Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures. Journal of Experimental Medicine, e20210012. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
30804752 | not listed | Modgil, A., et al. 2019. Neuroactive Steroids Reverse Tonic Inhibitory Deficits in Fragile X Syndrome Mouse Model. Frontier in Molecular Neuroscience, 15. |
29467616 | 1:500 | Everington, E.A., et al. 2018. Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress. Frontier in Molecular Neuroscience, 18. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
27060767 | 1:10 (supe) | Purgianto, A., et al. 2016. Surface expression of GABAA receptors in the rat nucleus accumbens is increased in early but not late withdrawal from extended-access cocaine self-administration. Brain Research, 336-343. |