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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged FGF13B/FHF2B, FGF13A/FHF2A or untagged Kv2.1 plasmid and probed with N225A/10 (left) or N86/8 (right) TC supe.
N56/21 immunofluorescence staining of cerebellar cortex from FGF14 wild-type (left) and knockout (right) mice. ml: molecular layer; pc: Purkinje cell layer; igl: internal granule cell layer; wm: white matter.Double immunofluorescence staining of cultured rat hippocampal neurons with N56/21 (red) and MAP2 (green). Images courtesy of David Ornitz (Washington University SOM).
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Transfected cell immunofluorescence: COS cells expressing Myc-tagged Flrt3. Red = Myc, Green = N353/13, Blue = Hoechst nuclear stain.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Foxi2 or untagged Kv2.1 plasmid and probed with N349/96 TC supe (left), Rabbit anti-Flag (middle) or N52A/42 TC supe (right.

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