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Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.Immunofluorescence staining of hippocampus from adult Kv1.1 wildtype (left) or knock-out (right) mouse with K36/15 (red) and K13/31 (green, Kv1.4).
Immunolabeling of postnatal 14 mouse hippocampus specifically labeling Kv1.1 (Cat no 75-007, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50 uM slices were floating sections, where antigen retrieval was performed with sodium citrate at 80 C for 30 minutes before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.Adult rat brain hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.
Adult rat dentate gyrus immunohistochemistry.Adult rat brain membrane immunoblot.

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