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Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).Adult rat cerebellum immunohistochemistry.
NeuroMab Anti-HCN1 Antibody
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Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).Adult rat hippocampus immunohistochemistry.
Adult rat retina membrane immunoblot (left) and immunofluorescence (right). Green = N114/10 staining of the inner nuclear, inner plexiform, ganglion cell and optic fiber layers (INL, IPL, GCL, and OFL), where layers are viewed by differential interference contrast microscopy. Data courtesy of Gloria Partida, Tyler Stradleigh, and Andrew Ishida (UCDavis).Adult rat thalamus immunohistochemistry.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N299/39 TC supe (left) and a mouse loading control (right).

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