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Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).Adult rat spinal cord dorsal horn immunohistochemistry.
Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).Transfected HEK293 cell immunofluorescence staining N15/39=green, DAPI=blue.
Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).
Immunoblot against extracts of COS cells transiently transfected with Myc/Flag-tagged TWIK-2 or untagged Kv2.1 plasmid probed with N469/28 (left) or rabbit anti-Flag (right)..

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