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Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274)..
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged VAPA, VAPB or untagged Kv2.1 plasmid probed with N479/107 (left) or N86/8 (right) TC supe..
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).

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