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Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue).  Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford)..Adult rat brain hippocampus immunohistochemistry.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228HEK293T transfectants expressing SLITRK1 or its mutants were co-cultured with hippocampal neurons. Two days after co-culture, induced pre-synapses were quantified through VGAT or VGLUT1 immunostaining and GFP. Image from publication CC-BY-4.0. PMID:36085162
NeuroMab Anti-VGlut1 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.

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